This study aimed to develop a quantitative PCR assay to simultaneously quantify human and dog DNA in a multispecies mixture to inform downstream analyses in routine forensic casework and scientific research. The human target is the Alu Yb8 element, which has approximately 2000 copies per cell, and the canine target is the SINEC_Cf element, which has approximately 200,000 copies per cell. The internal positive control is a universal exogenous assay consisting of forward and reverse primers, a NED-labeled probe with an MGBNFQ quencher and a 65-bp synthetic template. Results suggest a potentially robust assay with a fast run time and a high degree of sensitivity, precision and species specificity, with direct application to domestic pet samples in veterinary genetics and forensics.

中文翻译：

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用于同时检测和定量人类和狗 DNA 的基于短散布核元件的定量 PCR 测定。

本研究旨在开发一种定量 PCR 检测方法，以同时量化多物种混合物中的人和狗 DNA，为常规法医案件和科学研究中的下游分析提供信息。人类目标是Alu Yb8 元素，每个细胞大约有 2000 个拷贝，犬目标是 SINEC_Cf 元素，每个细胞大约有 200,000 个拷贝。内部阳性对照是一种通用外源检测，由正向和反向引物、带有 MGBNFQ 淬灭剂的 NED 标记探针和 65 bp 合成模板组成。结果表明，这是一种具有快速运行时间和高度灵敏度、精确度和物种特异性的潜在稳健分析，可直接应用于兽医遗传学和法医学中的家养宠物样本。