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A low-cost platform suitable for sequencing-based recovery of natural variation in understudied plants.
Biotechniques ( IF 2.7 ) Pub Date : 2020-12-14 , DOI: 10.2144/btn-2020-0132
Rachel Howard-Till 1, 2 , Claudia E Osorio 1, 3 , Bradley J Till 1, 4
Affiliation  

Genetic characterization of wild and cultivated plants provides valuable knowledge for conservation and agriculture. DNA sequencing technologies are improving, and costs are dropping. Yet analysis of many species is hindered because they grow in regions that lack infrastructure for advanced molecular biology. The authors developed and adapted low-cost methods that address these issues. Tissue was collected and stored in silica gel, avoiding the need for liquid nitrogen and freezers. The authors optimized low-cost, homemade DNA extraction to increase yields, reduce costs and produce DNA suitable for next-generation sequencing. The authors describe how to build a gel documentation system for DNA quantification. As a proof of principle, the authors used these methods to evaluate wild Berberis darwinii, native to Southern Chile.

中文翻译:

一种低成本平台,适用于对未充分研究植物的自然变异进行基于测序的恢复。

野生和栽培植物的遗传特征为保护和农业提供了宝贵的知识。DNA 测序技术正在改进,成本正在下降。然而,许多物种的分析受到阻碍,因为它们生长在缺乏先进分子生物学基础设施的地区。作者开发并调整了解决这些问题的低成本方法。收集组织并储存在硅胶中,避免使用液氮和冷冻机。作者优化了低成本、自制的 DNA 提取,以提高产量、降低成本并生产适合下一代测序的 DNA。作者描述了如何构建用于 DNA 定量的凝胶文档系统。作为原理证明,作者使用这些方法来评估野生小檗,原产于智利南部。
更新日期:2020-12-16
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