Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease worldwide. Rice introgression line H471, derived from the recurrent parent Huang-Hua-Zhan (HHZ) and the donor parent PSBRC28, exhibits broad-spectrum resistance to Xoo, including to the highly virulent Xoo strain PXO99A, whereas its parents are susceptible to PXO99A. To characterize the responses to Xoo, we compared the proteome profiles of the host and pathogen in the incompatible interaction (H471 inoculated with PXO99A) and the compatible interaction (HHZ inoculated with PXO99A). In this study, a total of 374 rice differentially abundant proteins (DAPs) and 117 Xoo DAPs were detected in the comparison between H471 + PXO99A and HHZ + PXO99A. Most of the Xoo DAPs related to pathogen virulence, including the outer member proteins, type III secretion system proteins, TonB-dependent receptors, and transcription activator-like effectors, were less abundant in the incompatible interaction than in the compatible interaction. The rice DAPs were mainly involved in secondary metabolic processes, including phenylalanine metabolism and the biosynthesis of flavonoids and phenylpropanoids. Additionally, some DAPs involved in the phenolic phytoalexin and salicylic acid (SA) biosynthetic pathways accumulated much more in H471 than in HHZ after the inoculation with PXO99A, suggesting that phytoalexin and SA productions were induced faster in H471 than in HHZ. Further analyses revealed that the SA content increased much more rapidly in H471 than in HHZ after the inoculation, suggesting that the SA signaling pathway was activated faster in the incompatible interaction than in the compatible interaction. Overall, our results indicate that during an incompatible interaction between H471 and PXO99A, rice plants prevent pathogen invasion and also initiate multi-component defense responses that inhibit disease development.

中文翻译：

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比较蛋白质组学分析揭示了水稻与米黄单胞菌之间的相互作用的新颖见解。水稻

细菌性枯萎病，是由米色黄单胞菌（Xanthomonas oryzae）PV引起的。稻米（Xoo）是世界范围内毁灭性的水稻疾病。水稻从回归亲本黄花展（HHZ）和供体亲本PSBRC28获得的基因渗入系H471对Xoo具有广谱抗性，包括对高毒力Xoo菌株PXO99A的抵抗力，而其亲本则对PXO99A敏感。为了表征对Xoo的反应，我们比较了不相容相互作用（用PXO99A接种的H471）和相容相互作用（用PXO99A接种的HHZ）中宿主和病原体的蛋白质组概况。在这项研究中，通过比较H471 + PXO99A和HHZ + PXO99A，共检测到374种水稻差异丰富蛋白（DAP）和117种Xoo DAP。大多数Xoo DAP与病原体毒力有关，包括外部成员蛋白，III型分泌系统蛋白，TonB依赖性受体和转录激活因子样效应物在不相容相互作用中比在相容相互作用中丰富。水稻DAPs主要参与次生代谢过程，包括苯丙氨酸代谢以及类黄酮和苯丙烷的生物合成。此外，接种PXO99A后，与酚类植物抗毒素和水杨酸（SA）生物合成途径有关的某些DAP在H471中的积累比在HHZ中积累的多得多，这表明H471诱导的植物抗毒素和SA的产生要比HHZ快。进一步的分析表明，接种后H471中SA含量的增加比HHZ中的增加要快得多，提示SA信号通路在不相容相互作用中的激活速度比在相容相互作用中的激活快。总体而言，我们的结果表明，在H471与PXO99A之间不兼容的相互作用期间，水稻植物可以防止病原体入侵，并且还可以启动抑制疾病发展的多组分防御反应。