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Evaluation of Reducing Ability and Antioxidant Activity of Fruit Pomace Extracts by Spectrophotometric and Electrochemical Methods
Journal of Analytical Methods in Chemistry ( IF 2.6 ) Pub Date : 2020-12-14 , DOI: 10.1155/2020/8869436
Georgii S. Vasyliev 1 , Victoria I. Vorobyova 1 , Olga V. Linyucheva 1
Affiliation  

The component profiles of apricot, grape, and black currant pomace extracts have been analyzed using HPLC coupled to diode-array detection and tandem mass spectrometry (HPLC-DAD-MS). The predominant components in grape, apricot, and black currant pomace extracts were phenolic acids and flavonols. The redox behavior of apricot, black currant, and grape pomace water extracts was evaluated by means of cyclic voltammetry. Also, individual substances mainly present in the extracts were analyzed. The results of electrochemical testing were compared to traditional chemical techniques of potassium ferricyanide reduction (FRAP) and phosphomolybdenum assay, and fair agreement was established. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assays were applied in order to estimate antioxidant activity. The reducing power of the grape extract was found to be higher than that of the apricot and black currant extracts in both potassium ferricyanide reduction (FRAP) and phosphomolybdenum methods.

中文翻译:

分光光度法和电化学法评价果渣提取物的还原能力和抗氧化活性

杏,葡萄和黑加仑果渣提取物的成分图已使用结合二极管阵列检测和串联质谱(HPLC-DAD-MS)的HPLC进行了分析。葡萄,杏和黑加仑果渣提取物中的主要成分是酚酸和黄酮醇。通过循环伏安法评估杏,黑加仑和葡萄渣水提取物的氧化还原行为。此外,分析了主要存在于提取物中的各种物质。将电化学测试的结果与传统的铁氰化钾还原(FRAP)和磷钼测定的化学技术进行比较,并建立了合理的协议。2,2-二苯基-1-picylhydrazyl(DPPH)自由基清除测定和2,为了估计抗氧化剂活性,使用了2'-叠氮基双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基清除法。在铁氰化钾还原法(FRAP)和磷钼法中,葡萄提取物的还原能力均高于杏和黑加仑提取物。
更新日期:2020-12-14
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