Exposure to pesticides is considered a major factor underlying increased risk of hematological disorders in agricultural workers due to its carcinogenic potential. However, genotoxic impact of pesticides in DNA integrity of bone marrow stem cells (BMSC) of farmers exposed is not yet well known. We evaluated presence of chromosomal abnormalities (CA) and mRNA expression of DNA repair targets (ATM, BRCA1, BRCA2, RAD51, XRCC5, XRCC6, LIG4, CSA, CSB, XPA, XPC, XPG) in 90 bone marrow samples of farmers divided into three groups: commercial farming (CF), family farming (FF) and organic farming (OF). Our results showed that farmers in CF (72.7 %) and FF (27.3 %) groups had significantly higher values of CA when compared to OF group (0.0 %; p = 0.003). CF showed lower XPG (p = 0.008), CSA (p < 0.001), ATM (p = 0.036) and LIG4 (p = 0.004) mRNA expression than OF. FF presented lower XPG (p = 0.012) and LIG4 (p = 0.004) expression than OF. CF + FF individual with ≥12 years of exposure to pesticides showed decreased mRNA expression of XPC (p = 0.001), XPG (p = 0.010), CSB (p = 0.05), ATM (p = 0.030) and LIG4 (p = 0.044) than those who have been exposed for <12 years. CF + FF with CA showed a lower expression of BRCA2 when compared to CF + FF group without CA (p = 0.007). These results highlight that genotoxic exposure to pesticides negatively affects expression profile of important DNA repair genes in BMSC, favoring irreparable chromosomal lesions.

由于其潜在的致癌性，农药暴露被认为是增加农业工人血液系统疾病风险的主要因素。然而，农药对暴露的农民的骨髓干细胞（BMSC）DNA完整性的遗传毒性影响尚不为人所知。我们评价染色体异常的存在（CA）和mRNA表达的DNA修复的目标（ATM，BRCA1，BRCA2，RAD51，XRCC5，XRCC6，LIG4，CSA，CSB，XPA，XPC，XPG）在90个骨髓样本中将农民分为三类：商业农业（CF），家庭农业（FF）和有机农业（OF）。我们的结果表明，与OF组（0.0％； p = 0.003）相比，CF（72.7％）和FF（27.3％）组的农民具有较高的CA值。CF显示的XPG（p = 0.008），CSA（p <0.001），ATM（p = 0.036）和LIG4（p = 0.004）mRNA的表达均低于OF。FF的XPG（p = 0.012）和LIG4（p = 0.004）的表达低于OF。接触农药≥12年的CF + FF患者显示XPC（p = 0.001），XPG（p = 0.010），CSB的mRNA表达降低（p = 0.05），ATM（p = 0.030）和LIG4（p = 0.044）的暴露时间少于<12年。与没有CA的CF + FF组相比，具有CA的CF + FF显示较低的BRCA2表达（p = 0.007）。这些结果表明，农药的遗传毒性暴露会对BMSC中重要的DNA修复基因的表达谱产生负面影响，有利于不可修复的染色体损伤。