Our lineage tracing studies using multiple Cre mouse lines showed a concurrent labeling of abundant taste bud cells and the underlying connective tissue with a neural crest (NC) origin, warranting a further examination on the issue of whether there is an NC derivation of taste bud cells. In this study, we mapped NC cell lineages in three different models, Sox10-iCreER^T2/tdT mouse, GFP⁺ neural fold transplantation to GFP⁻ chickens, and Sox10-Cre/GFP-RFP zebrafish model. We found that in mice, Sox10-iCreER^T2 specifically labels NC cell lineages with a single dose of tamoxifen at E7.5 and that the labeled cells were widely distributed in the connective tissue of the tongue. No labeled cells were found in taste buds or the surrounding epithelium in the postnatal mice. In the GFP⁺/GFP⁻ chicken chimera model, GFP⁺ cells migrated extensively to the cranial region of chicken embryos ipsilateral to the surgery side but were absent in taste buds in the base of oral cavity and palate. In zebrafish, Sox10-Cre/GFP-RFP faithfully labeled known NC-derived tissues but did not label taste buds in lower jaw or the barbel. Our data, together with previous findings in axolotl, indicate that taste buds are not derived from NC cells in rodents, birds, amphibians or teleost fish.

我们使用多个Cre小鼠系的谱系追踪研究显示，同时标记大量味蕾细胞和具有神经嵴 (NC) 起源的潜在结缔组织，需要进一步检查味蕾细胞是否存在 NC 衍生问题. 在这项研究中，我们在三种不同的模型中绘制了 NC 细胞谱系图，即 Sox10-iCreER ^T2 / tdT小鼠、GFP ⁺神经褶皱移植到 GFP ^-鸡和Sox10-Cre/GFP-RFP斑马鱼模型。我们发现在小鼠中，Sox10-iCreER ^T2用单剂量的他莫昔芬在 E7.5 专门标记 NC 细胞谱系，并且标记的细胞广泛分布在舌头的结缔组织中。在产后小鼠的味蕾或周围上皮中未发现标记细胞。在 GFP ⁺ /GFP ^-鸡嵌合体模型中，GFP ⁺细胞广泛迁移到手术侧同侧鸡胚胎的颅骨区域，但在口腔和上颚底部的味蕾中不存在。在斑马鱼中，Sox10-Cre/GFP-RFP忠实地标记了已知的 NC 衍生组织，但没有标记下颚或触须的味蕾。我们的数据以及之前在蝾螈中的发现表明，味蕾并非来自啮齿动物、鸟类、两栖动物或硬骨鱼的 NC 细胞。