Clonal anergy and depletion of antigen-specific CD8+ T cells are characteristics of immunosuppressed patients such as cancer and post-transplant patients. This has promoted translational research on the adoptive transfer of T cells to restore the antigen-specific cellular immunity in these patients. In the present work, we compared the capability of PBMCs and two types of mature monocyte-derived DCs (moDCs) to prime and to expand ex-vivo antigen-specific CD8+ T cells using culture conditioned media supplemented with IL-7, IL-15, and IL-21. The data obtained suggest that protocols involving moDCs are as efficient as PBMCs-based cultures in expanding antigen-specific CD8+ T cell to ELA and CMV model epitopes. These three gamma common chain cytokines promote the expansion of naïve-like and central memory CD8+ T cells in PBMCs-based cultures and the expansion of effector memory T cells when moDCs were used. Our results provide new insights into the use of media supplemented with IL-7, IL-15, and IL-21 for the in-vitro expansion of early-differentiated antigen-specific CD8+ T cells for immunotherapy purposes.

克隆性无反应和抗原特异性CD8 + T细胞的耗竭是免疫抑制患者（如癌症和移植后患者）的特征。这促进了对T细胞过继转移以恢复这些患者的抗原特异性细胞免疫的翻译研究。在目前的工作中，我们比较了PBMC和两种类型的成熟单核细胞衍生DC（moDC）引发和扩展体外能力的能力。使用补充有IL-7，IL-15和IL-21的培养条件培养基培养抗原特异性CD8 + T细胞。获得的数据表明，涉及moDC的方案在将抗原特异性CD8 + T细胞扩展至ELA和CMV模型表位方面与基于PBMC的培养一样有效。在基于PBMC的培养物中，这三种γ共有链细胞因子促进幼稚样和中央记忆CD8 + T细胞的扩增，并在使用moDC时促进效应记忆T细胞的扩增。我们的结果提供了对于使用补充有IL-7，IL-15和IL-21的培养基进行体外分化的早期分化抗原特异性CD8 + T细胞进行免疫治疗的新见解。