High resolution melting (HRM) is a fast closed-tube method for nucleotide variant scanning applicable for bacterial species identification or molecular typing. Recently a novel HRM-based method for Klebsiella pneumoniae typing has been proposed: it consists of an HRM protocol designed on the capsular wzi gene and an HRM-based algorithm of strains clustering. In this study, we evaluated the repeatability and reproducibility of this method by performing the HRM typing of a set of K. pneumoniae strains, on three different instruments and by two different operators. The results showed that operators do not affect melting temperatures while different instruments can. Despite this, we found that strain clustering analysis, performed using MeltingPlot separately on the data from the three instruments, remains almost perfectly consistent. The HRM method under study resulted highly repeatable and thus reliable for large studies, even when several operators are involved. Furthermore, the HRM clusters obtained from the three different instruments were highly conserved, suggesting that this method could be applied in multicenter studies, even if different instruments are used.

高分辨率熔解（HRM）是用于核苷酸变异扫描的快速封闭管方法，适用于细菌种类鉴定或分子分型。最近，有人提出了一种新的基于HRM的肺炎克雷伯菌分型方法：它由在荚膜wzi基因上设计的HRM方案和基于HRM的菌株聚类算法组成。在这项研究中，我们通过对一组肺炎克雷伯菌进行HRM分型，评估了该方法的可重复性和可重复性。应变，在三种不同的仪器上并由两名不同的操作员进行。结果表明，操作员不会影响熔融温度，而不同的仪器却可以。尽管如此，我们发现，使用MeltingPlot分别对三种仪器的数据进行的应变聚类分析仍然几乎完全一致。即使在涉及多个操作员的情况下，所研究的HRM方法也具有高度可重复性，因此对于大型研究而言是可靠的。此外，从三种不同仪器获得的HRM簇是高度保守的，这表明即使使用不同的仪器，该方法也可以应用于多中心研究。