Synthetic fungicides are eco-unfriendly to agriculture and the environment. Agricultural Jiaosu (AJ), which originates from organic wastes, has the potential to be a substitute for synthetic fungicides. In this study, the characteristics of AJ and its antifungal activity against Botrytis cinerea were investigated for the first time. AJ was rich in lactic acid (4.46 g/L), acetic acid (1.52 g/L), Lactobacillus (72.45%) and Acetobacter (15.23%), which was a microbial ecosystem consisting of acid-based substances (AS) and beneficial microorganisms (BM). The results of the antifungal assays suggested that B. cinerea was effectively inhibited by AJ, with the half-maximal inhibitory concentration (IC₅₀) of 9.24%. AJ showed the strongest and most-lasting inhibitory effect compared to cell-free supernatant and microbial solution of AJ, indicating that AS and BM and their synergistic effect contributed to the antifungal activity of AJ. Two-step inhibition’ is an antifungal mode of AJ. Firstly, AS not only inhibited the pathogen directly but also provided a dominant niche for BM of AJ. Then, BM in AJ, especially Acetobacter, proliferated and metabolized acetic acid continuously. Thus, AJ achieved high-efficiency and long-acting inhibition. AJ is a promising biological agent considering its features of an eco-friendly, low-cost and easy-to-operate biological agent in rural areas.

合成杀真菌剂对农业和环境不利于生态。来自有机废物的农业焦素（AJ）有潜力替代合成杀真菌剂。在本研究中，首次研究了AJ的特性及其对灰葡萄孢的抗真菌活性。AJ富含乳酸（4.46 g / L），乙酸（1.52 g / L），乳酸杆菌（72.45％）和醋杆菌（15.23％），这是一个由酸类物质（AS）和有益生物组成的微生物生态系统微生物（BM）。抗真菌试验的结果表明，灰黄芽孢杆菌可被AJ有效抑制，抑制浓度为半数最大浓度（IC ₅₀）的9.24％。与无细胞上清液和微生物溶液相比，AJ表现出最强和最持久的抑制作用，表明AS和BM及其协同作用有助于AJ的抗真菌活性。“两步抑制”是AJ的抗真菌模式。首先，AS不仅直接抑制病原菌，而且为AJ的BM提供了优势地位。然后，AJ中的BM（尤其是醋杆菌）不断增殖并代谢乙酸。因此，AJ获得了高效率和长效的抑制作用。考虑到其在农村地区的生态友好，低成本和易于操作的特性，AJ是一种很有前途的特性。