A 6‐week feeding trial was conducted to investigate the effects of adding tributyrin (TB), alone and in combination with fructooligosaccharides (FOS) in high soybean meal (SBM) diets on growth performance, innate immunity and intestinal morphology of shrimp (Litopenaeus vannamei). Six isonitrogenous and isolipidic diets were formulated: positive control diet (PC, containing 250 g/kg fishmeal (FM) and 285 g/kg SBM), control diet (C, containing 125 g/kg FM and 439.2 g/kg SBM) and four experimental diets with 10 g/kg TB (T1), 10 g/kg TB + 1.0 g/kg FOS (T2), 10 g/kg TB + 2.0 g/kg FOS (T3) and 10 g/kg TB + 4.0 g/kg FOS (T4) supplemented to the control diet. The results showed that shrimps fed diet C had the poorest growth, and no significant differences in growth performance and survival rates were observed in shrimps fed diets PC, T1, T3 and T4. Dietary TB supplementation significantly enhanced hepatopancreas trypsin, haemolymph ACP and AKP activities and also improved mucosal thickness (MT) (p < .05). Compared with supplementation of TB alone, the combination of TB and FOS not only further improved the above indicators but also enhanced the villus height (VH), hepatopancreas lipase and haemolymph SOD activities, while hepatopancreas MDA level decreased significantly (p < .05), especially in groups with higher doses of FOS (2 and 4 g/kg). In conclusion, partial FM replacement with SBM had negative effects on shrimp, and TB alone and in combination with FOS can improve the growth performance, innate immunity and maintain healthy intestinal morphology of shrimps fed with high SBM diets.

中文翻译：

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在虾的高豆粕日粮中单独添加或与果寡糖合用三丁酸甘油酯（对虾）：对生长，先天免疫和肠道形态的影响

进行了为期6周的喂养试验，以研究在高豆粕（SBM）日粮中单独添加三丁酸（TB）以及与低聚果糖（FOS）联合添加对虾的生长性能，先天免疫力和肠道形态的影响）。配制了六种等氮和等脂日粮：阳性对照日粮（PC，包含250 g / kg鱼粉（FM）和285 g / kg SBM），对照日粮（C，包含125 g / kg FM和439.2 g / kg SBM）和四种实验饮食，分别为10 g / kg TB（T1），10 g / kg TB + 1.0 g / kg FOS（T2），10 g / kg TB + 2.0 g / kg FOS（T3）和10 g / kg TB + 4.0 g / kg FOS（T4）补充对照饮食。结果表明，饲喂日粮C的虾生长最差，饲喂日粮PC，T1，T3和T4的虾的生长性能和成活率没有显着差异。饮食中补充结核病可显着增强肝胰脏胰蛋白酶，血淋巴ACP和AKP活性，并改善粘膜厚度（MT）（p <.05）。与单独补充结核相比，结核和FOS的结合不仅进一步改善了上述指标，而且还增强了绒毛高度（VH），肝胰腺脂肪酶和血淋巴SOD活性，而肝胰腺MDA水平显着降低（p  <.05），特别是在FOS剂量较高（2和4 g / kg）的人群中。总之，用SBM替代部分FM对虾有负面影响，单独使用TB和FOS联合使用TB可以改善高SBM饲喂对虾的生长性能，先天免疫力并保持健康的肠道形态。