Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term activity patterns for both hungry and satiated flies in conditions of foraging remain to be better understood. Similarly, little is known about how chronic contact chemosensory stimulation affects locomotion. To address these questions, we have developed a novel, simplistic fly activity tracking setup - the Panopticon. Using a 3D-printed Petri dish inset, our assay allows recording of walking behaviour, of several flies in parallel, with all arena surfaces covered by a uniform substrate layer. We tested two constellations of providing food: i) in single patches, and ii) omnipresent within the substrate layer. Fly tracking is done with FIJI, further assessment, analysis and presentation is done with a custom-built MATLAB analysis framework. We find that starvation history leads to a long-lasting reduction in locomotion, as well as a delayed place preference for food patches not driven by immediate hunger motivation.

中文翻译：

---

饥饿经验对果蝇活动和位置偏好的长期影响

动物的行为明显受感觉刺激，以前的经历和诸如饥饿之类的内部状态支配。随着饥饿的增加，优先重点转向觅食和觅食。在觅食期间，果蝇会采用有效的路径整合策略。然而，觅食条件下饥饿和饱腹蝇的一般长期活动模式仍有待更好地理解。同样，对慢性接触化学感觉刺激如何影响运动知之甚少。为了解决这些问题，我们开发了一种新颖，简单的飞行活动跟踪装置-Panopticon。使用3D打印的陪替氏培养皿插图，我们的测定方法可以记录平行飞行的几只苍蝇的步行行为，且所有竞技场表面均被均匀的基底层覆盖。我们测试了两个提供食物的星座：i）在单个贴片中，和ii）在基质层中无处不在。使用FIJI进行飞行跟踪，使用定制的MATLAB分析框架进行进一步的评估，分析和演示。我们发现饥饿的历史导致运动的长期减少，以及对不是由眼前饥饿动机驱动的食物的偏爱。