Enhancement of the desulfurization activities of Paenibacillus strains 32O-W and 32O-Y were investigated using dibenzothiophene (DBT) and DBT sulfone (DBTS) as sources of sulfur in growth experiments. Strains 32O-W, 32O-Y and their co-culture (32O-W plus 32O-Y), and Vitreoscilla hemoglobin (VHb) expressing recombinant strain 32O-Yvgb and its co-culture with strain 32O-W were grown at varying concentrations (0.1-2 mmol L-1 ) of DBT or DBTS for 96 h, and desulfurization measured by production of 2-hydroxybiphenyl (2-HBP) and disappearance of DBT or DBTS. Of the four cultures grown with DBT as sulfur source, the best growth occurred for the 32O-Yvgb plus 32O-W co-culture at 0.1 and 0.5 mmol L-1 DBT. Although the presence of vgb provided no consistent advantage regarding growth on DBTS, strain 32O-W, as predicted by previous work, was shown to contain a partial 4S desulfurization pathway allowing it to metabolize this 4S pathway intermediate.

中文翻译：

---

结合类芽孢杆菌菌株和玻璃体血红蛋白表达的共培养作为改善生物脱硫的策略

使用二苯并噻吩 (DBT) 和 DBT 砜 (DBTS) 作为生长实验中的硫源，研究了类芽孢杆菌菌株 32O-W 和 32O-Y 脱硫活性的增强。菌株 32O-W、32O-Y 及其共培养物（32O-W 加 32O-Y）和表达重组菌株 32O-Yvgb 的玻璃体血红蛋白 (VHb) 及其与菌株 32O-W 的共培养物以不同浓度生长(0.1-2 mmol L-1 ) DBT 或 DBTS 96 小时，脱硫通过 2-羟基联苯 (2-HBP) 的产生和 DBT 或 DBTS 的消失来测量。在以 DBT 作为硫源生长的四种培养物中，32O-Yvgb 加 32O-W 共培养物在 0.1 和 0.5 mmol L-1 DBT 下生长最好。尽管 vgb 的存在对 DBTS 的生长没有提供一致的优势，菌株 32O-W，如先前工作所预测的，