Despite several studies on fish hormone therapy, finding new candidates may provide more reproductive efficiency in artificial propagation. Kisspeptins, being upstream of the hypothalamic-pituitary-gonadal axis, appear to play a key role in the reproduction process. In the present study, the effect of different variants of kiss peptide including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss), and their combination (Kiss1 + H) was investigated on the reproductive indices of goldfish broodstock in comparison to Ovaprim (a typical synthetic Gnrh hormone). Peptides (Kiss1 and Hkiss) were synthesized using a solid-phase synthesis approach. Kiss1 and Hkiss were injected at a dose of 100 μg/kg body weight, blood samples were taken six hours after injection and sex hormones (E2, Dhp, and 11-Kt), gonadotropins (Lh and Fsh), cortisol, and reproductive indices (fecundity, fertilization, and hatching percentage) were measured. The results showed a significant increase of plasma sex hormones and gonadotropins in fish treated with kisspeptins. In addition, the cortisol and lipoprotein lipase in Kiss1, Hkiss, and Kiss1 + H were remarkably increased compared to Ovaprim. In conclusion, kisspeptins could be a more suitable candidate than Ovaprim for accelerating and synchronizing oocyte maturation in the fisheries industry. This article is protected by copyright. All rights reserved.

中文翻译：

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不同外源kisspeptins对金鱼(Carassius auratus)亲鱼性激素和繁殖指标的影响

尽管对鱼类激素疗法进行了多项研究，但寻找新的候选药物可能会在人工繁殖中提供更高的繁殖效率。Kisspeptins 位于下丘脑-垂体-性腺轴的上游，似乎在生殖过程中起着关键作用。本研究比较了金鱼（Carassius auratus）kisspeptin（Kiss1）、人kisspeptin（Hkiss）及其组合（Kiss1+H）等不同亲吻肽变体对金鱼亲鱼繁殖指标的影响。到 Ovaprim（一种典型的合成 Gnrh 激素）。使用固相合成方法合成肽（Kiss1 和 Hkiss）。Kiss1 和 Hkiss 以 100 μg/kg 体重的剂量注射，注射后 6 小时采集血样，性激素（E2、Dhp 和 11-Kt）、促性腺激素（Lh 和 Fsh）、测量了皮质醇和生殖指数（繁殖力、受精率和孵化率）。结果显示用kisspeptins处理的鱼的血浆性激素和促性腺激素显着增加。此外，与 Ovaprim 相比，Kiss1、Hkiss 和 Kiss1 + H 中的皮质醇和脂蛋白脂肪酶显着增加。总之，在渔业行业中，kisspeptins 可能是比 Ovaprim 更适合加速和同步卵母细胞成熟的候选者。本文受版权保护。版权所有。和 Kiss1 + H 与 Ovaprim 相比显着增加。总之，在渔业行业中，kisspeptins 可能是比 Ovaprim 更适合加速和同步卵母细胞成熟的候选者。本文受版权保护。版权所有。和 Kiss1 + H 与 Ovaprim 相比显着增加。总之，在渔业行业中，kisspeptins 可能是比 Ovaprim 更适合加速和同步卵母细胞成熟的候选者。本文受版权保护。版权所有。