In winter and summer of 2016 and 2017, airborne fungi and house dust were collected in indoors of the village Gunja, which had been flooded, and the control village Gornji Stupnik (Croatia) in order to explore variations of fungal indoor levels, particularly Aspergilli section Nidulantes series Versicolores, as well as fungal metabolites in dust. Levels of airborne Aspergilli (Versicolores) were three times as high in winter and summer in Gunja than in the control village, while dustborne isolates were equally present in both locations. Sequencing of the calmodulin gene region revealed that among Aspergilli (Versicolores), A. jensenii and A. creber were dominant and together with A. puulaauensis, A. tennesseensis and A. venenatus produced sterigmatocystin and 5‐methoxysterigmatocystin (HPLC coupled with mass spectrometry); A. amoenus, A. fructus, A. griseoaurantiacus, A. pepii, and A. protuberus produced sterigmatocystin but not 5‐methoxysterigmatocystin; A. sydowii did not produce any of these toxins. A total of 75 metabolites related to Penicillium (29), Aspergillus (22), Fusarium (10), Alternaria (5), Stachybotrys (2), and other fungi (7) were detected in dust by liquid chromatography‐tandem mass spectrometry. The majority of metabolites including sterigmatocystin and 5‐methoxysterigmatocystin exhibited a higher prevalence in winter in Gunja.

中文翻译：

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克罗地亚东部发生重大洪水事件后，室内受水破坏的真菌及其次生代谢产物

在 2016 年和 2017 年的冬夏，在被洪水淹没的 Gunja 村和控制村 Gornji Stupnik（克罗地亚）的室内收集了空气中的真菌和屋尘，以探索室内真菌水平的变化，特别是曲霉部分Nidulantes系列Versicolores，以及灰尘中的真菌代谢物。Gunja 冬季和夏季的空气中曲霉 ( Versicolores ) 水平是对照村庄的三倍，而两个地点的尘传分离株同样存在。钙调素基因区域的测序显示，在曲霉菌（Versicolores）中，A. jensenii和A. crerber占优势，与A. puulaauensis、A. tennesseensis和A. venenatus产生 sterigmatocystin 和 5-甲氧基 sterigmatocystin（HPLC 与质谱联用）；A. amoenus，A.枸杞，A. griseoaurantiacus，A. pepii，和A. protuberus产生杂色曲霉素但不是5- methoxysterigmatocystin; A. sydowii不产生任何这些毒素。共有 75 种代谢物与青霉(29)、曲霉(22)、镰刀菌(10)、链格孢(5)、葡萄孢属相关(2) 和其他真菌 (7) 通过液相色谱-串联质谱法在灰尘中检测到。大多数代谢物，包括 sterigmatocystin 和 5-甲氧基 sterigmatocystin 在 Gunja 的冬季表现出较高的流行率。