A microbial fuel cell (MFC) system containing modular half‐submerged biocathode was operated for 6 months in an 800 L flow‐through system with domestic wastewater. For the first time, spatial and temporal differences in biofilm communities were examined on large three‐dimensional electrodes in a wastewater MFC. Biocathode microbial community analysis showed a specialized biofilm community with electrogenic and electrotrophic taxa forming during operation, suggesting potentially opposing electrode reactions. The anodic community structure shifted during operation, but no spatial differences were observed along the length of the electrode. Power output from the system was most strongly influenced by pH. Higher power densities were associated with the use of solids‐dewatering filtrate with increased organic matter, conductivity, and pH. The results show that the biocathode was the rate‐limiting step and that future MFC design should consider the effect of size, shape, and orientation of biocathodes on their community assembly and electrotrophic ability.

中文翻译：

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高档生物阴极废水微生物燃料电池的生物膜结构、动力学和生态学

包含模块化半浸没式生物阴极的微生物燃料电池 (MFC) 系统在 800 L 的生活废水流通系统中运行了 6 个月。首次在废水 MFC 中的大型三维电极上检查了生物膜群落的时空差异。生物阴极微生物群落分析显示，在操作过程中形成了一个特殊的生物膜群落，具有产电和养电类群，表明可能存在相反的电极反应。阳极群落结构在操作过程中发生了变化，但沿电极长度没有观察到空间差异。系统的功率输出受 pH 值的影响最大。更高的功率密度与使用增加有机物、电导率和 pH 值的固体脱水滤液有关。