Yellow drum juveniles were fed with graded n‐3 long‐chain polyunsaturated fatty acids (n‐3 LC‐PUFAs) diets (1.5–15.9 g/kg) for ten weeks. Fish growth performance was firstly promoted then inhibited by increased n‐3 LC‐PUFAs inclusion. The optimum n‐3 LC‐PUFAs were 9.0 g kg⁻¹ and 9.5 g kg⁻¹, referring to the weight gain rate and feed efficiency ratio, respectively. The increased n‐3 LC‐PUFAs inclusion significantly decreased ash and moisture content but increased crude lipid content (p < .05). No significant alteration in fish protein content was observed among groups. Significantly higher saturated fatty acids proportion but the lower arachidonic acid proportion was observed in the subcutaneous adipose tissue (SCAT) of fish fed the diet with optimum n‐3 LC‐PUFAs group (9.8 g kg⁻¹), compared with those of the control group (1.5 g kg⁻¹) (p < .05). Transcriptome analysis found differently expressed genes (DEGs) were mostly enriched in the peroxisome and fatty acid biosynthesis pathways. Besides, the expression of an immune‐related gene, Interleukin‐17D was observed to significantly changed (p < .05). Therefore, 9.0–9.5 g kg⁻¹ n‐3 LC‐PUFAs could be regarded as optimum for the yellow drum. Dietary n‐3 LC‐PUFAs affected the SCAT some metabolism and immunity genes expression.

中文翻译：

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日粮n-3长链多不饱和脂肪酸（n-3 LC-PUFAs）对少年黄鼓鱼（Nibea albiflora）生长性能，身体成分和皮下脂肪组织转录组分析的影响

向黄鼓幼鱼喂食分级n-3长链多不饱和脂肪酸（n-3 LC-PUFAs）日粮（1.5-15.9 g / kg）十周。首先提高了鱼类的生长性能，然后通过增加n-3 LC-PUFAs的含量来抑制鱼类的生长。最佳的n-3 LC-PUFA分别为9.0 g kg ^-1和9.5 g kg ^-1，分别指增重率和进料效率比。增加的n-3 LC-PUFA夹杂物显着降低了灰分和水分含量，但增加了粗脂质含量（p <.05）。各组之间未观察到鱼蛋白质含量的显着变化。与对照组相比，n-3 LC-PUFAs最佳组（9.8 g kg ^-1）喂养的鱼的皮下脂肪组织（SCAT）中饱和脂肪酸比例明显较高，但花生四烯酸比例较低。组（1.5g kg ^-1）（p  ＜.05）。转录组分析发现不同表达的基因（DEGs）大多富含过氧化物酶体和脂肪酸的生物合成途径。此外，观察到免疫相关基因白细胞介素17D的表达发生了显着变化（p  <.05）。因此，9.0–9.5 g千克^-1n-3 LC-PUFA可能被认为是黄色鼓的最佳选择。日粮n-3 LC-PUFAs影响了SCAT的某些代谢和免疫基因的表达。