Abstract This work describes the synthesis of sebacic acid capped silver nanoparticles (SA-AgNPs) by the wet chemical method for the detection of L-Histidine in blood serum and urine samples. The synthesized SA-AgNPs were characterized by several techniques. The obtained particles were spherical in shape and the average diameter was calculated by HR-TEM images to be 11 nm. Moreover, the synthesized SA-AgNPs were stable for more than 3 months in solution. The synthesized SA-AgNPs showed the surface plasmon resonance (SPR) band at 420 nm. Interestingly, after the addition of 18.1 μM of L-Histidine into the SA-AgNPs solution, the SPR band absorbance decreased and the colour of the solution changed from brown to colourless. Based on the changes in the SPR band, we calculated the lowest detection limit to be 122 nM. The observed colour shift and spectral changes were due to the aggregation of SA-AgNPs induced by L-Histidine via hydrogen bonding interaction. Additionally, a possible mechanism is discussed. Furthermore, a 268-fold excess of common interferences did not interfere with the detection of 18.1 μM of L-Histidine. We successfully applied the SA-AgNPs probe for the detection of L-Histidine in blood serum and urine samples. Further, we validated our system with the HPLC technique. Importantly, we prepared a portable smartphone aided paper-based kit for on-site monitoring of L-Histidine. We strongly hope that this facile synthesis of SA-AgNPs and the portable paper-based kit will represent a step forward in the field of biosensing and materials science.

中文翻译：

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使用癸二酸封端的银纳米颗粒制备便携式量热试剂盒和一步分光光度法纳摩尔水平检测血清和尿液样品中的 L-组氨酸

摘要 这项工作描述了用湿化学法合成癸二酸封端的银纳米颗粒 (SA-AgNPs)，用于检测血清和尿液样品中的 L-组氨酸。合成的 SA-AgNPs 通过多种技术进行表征。所得颗粒为球形，通过HR-TEM图像计算平均直径为11nm。此外，合成的 SA-AgNPs 在溶液中稳定超过 3 个月。合成的 SA-AgNPs 在 420 nm 处显示出表面等离子体共振 (SPR) 带。有趣的是，在 SA-AgNPs 溶液中加入 18.1 μM L-组氨酸后，SPR 带吸光度降低，溶液颜色从棕色变为无色。根据 SPR 波段的变化，我们计算出最低检测限为 122 nM。观察到的色移和光谱变化是由于 L-组氨酸通过氢键相互作用诱导的 SA-AgNPs 的聚集。此外，还讨论了一种可能的机制。此外，268 倍过量的常见干扰物不会干扰 18.1 μM L-组氨酸的检测。我们成功地将 SA-AgNPs 探针用于检测血清和尿液样本中的 L-组氨酸。此外，我们使用 HPLC 技术验证了我们的系统。重要的是，我们准备了一个便携式智能手机辅助纸质套件，用于现场监测 L-组氨酸。我们强烈希望这种简单的 SA-AgNPs 合成和便携式纸质试剂盒将代表生物传感和材料科学领域向前迈出的一步。