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Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2020-12-11 , DOI: 10.1016/s2095-3119(20)63190-2
Yuan CHEN , Zhen-yu LIU , Leila I.M. TAMBEL , Xiang ZHANG , Yuan CHEN , De-hua CHEN

To clarify the effect of the N deficit on the amount of square Bt insecticidal protein, different N application rates (0, 75, 150, 225, and 300 kg ha–1) were imposed on the conventional cultivar Sikang 1 (SK-1) and hybrid cultivar Sikang 3 (SK-3) during 2015–2016 cotton growth seasons. Under different N application rates, the square number per plant, square volume and square dry weight reduced when the N rates decreased from conventional rate (300 kg ha–1) to 0 kg ha–1. And the square Bt protein content decreased accordingly. The analysis of N metabolism showed that soluble protein content, GPT and GOT activities decreased, free amino acid, peptidase and protease activities increased under N deficit. Correlation analysis indicated that the reduced Bt protein content under N deficit was related to altered N metabolism. In conclusion, square development and the amount of square Bt toxin both decreased under N deficit, indicating that promoting the square development under appropriate N application rate would also promote the insect resistance during squaring stage.



中文翻译:

氮不足下转基因棉花苏云金芽孢杆菌杀虫蛋白含量降低

为了阐明氮素不足对方形Bt杀虫蛋白含量的影响,对传统品种Sikang 1(SK-1)施用了不同的氮肥施用量(0、75、150、225和300 kg ha –1)。在2015–2016年棉花生长季节中使用了杂交种Sikang 3(SK-3)。在不同的氮施用量下,当氮的施用量从常规施用量(300 kg ha –1)降至0 kg ha –1时,每株植物的平方数,平方体积和平方干重降低。。并且正方形Bt蛋白含量相应地降低。氮素代谢分析表明,氮素亏缺时可溶性蛋白质含量,GPT和GOT活性降低,游离氨基酸,肽酶和蛋白酶活性增加。相关分析表明,氮缺乏时Bt蛋白含量降低与氮代谢改变有关。综上所述,在氮不足的情况下,正方形的发育和正方形Bt毒素的含量均下降,这表明在适当的氮肥施用量下促进正方形的发育也将促进平方期间的抗虫性。

更新日期:2020-12-13
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