Soil water deficit and salt stress are major limiting factors of plant growth and agricultural productivity. The primary root is the first organ to perceive the stress signals for drought and salt stress. In this study, maize plant subjected to drought, salt and combined stresses displayed a significantly reduced primary root length relative to the control plants. GC-MS was used to determine changes in the metabolites of the primary root of maize in response to salt, drought and combined stresses. A total of 86 metabolites were measured, including 29 amino acids and amines, 21 organic acids, four fatty acids, six phosphoric acids, 10 sugars, 10 polyols, and six others. Among these, 53 metabolites with a significant change under different stresses were identified in the primary root, and the content of most metabolites showed down-accumulation. A total of four and 18 metabolites showed significant up- and down-accumulation to all three treatments, respectively. The levels of several compatible solutes, including sugars and polyols, were increased to help maintain the osmotic balance. The levels of metabolites involved in the TCA cycle, including citric acid, ketoglutaric acid, fumaric acid, and malic acid, were reduced in the primary root. The contents of metabolites in the shikimate pathway, such as quinic acid and shikimic acid, were significantly decreased. This study reveals the complex metabolic responses of the primary root to combined drought and salt stresses and extends our understanding of the mechanisms involved in root responses to abiotic tolerance in maize.

土壤缺水和盐胁迫是植物生长和农业生产力的主要限制因素。第一根是第一个感知干旱和盐胁迫胁迫信号的器官。在这项研究中，与对照植物相比，遭受干旱，盐和综合胁迫的玉米植株的初生根长度显着减少。GC-MS用于确定玉米原根代谢产物在盐，干旱和综合胁迫下的变化。总共测量了86种代谢产物，包括29种氨基酸和胺，21种有机酸，4种脂肪酸，6种磷酸，10种糖，10种多元醇和其他6种。其中，在原始根中鉴定出53种在不同胁迫下变化显着的代谢物，大多数代谢物的含量均显示出向下积累。总共四种和18种代谢物分别对所有三种处理均显示出明显的上，下累积。增加了包括糖和多元醇在内的几种相容性溶质的含量，以帮助维持渗透平衡。TCA循环中涉及的代谢物水平（包括柠檬酸，酮戊二酸，富马酸和苹果酸）在主根中降低了。iki草酸途径中的代谢产物如奎尼酸和sh草酸的含量显着降低。这项研究揭示了原始根对干旱和盐胁迫综合的复杂代谢反应，并扩展了我们对玉米对非生物耐受性的根反应所涉及的机制的理解。增加了包括糖和多元醇在内的几种相容性溶质的含量，以帮助维持渗透平衡。TCA循环中涉及的代谢物水平（包括柠檬酸，酮戊二酸，富马酸和苹果酸）在主根中降低了。iki草酸途径中的代谢产物如奎尼酸和sh草酸的含量显着降低。这项研究揭示了原始根对干旱和盐胁迫的综合代谢反应，并扩展了我们对玉米对非生物耐受性的根反应所涉及的机制的理解。增加了包括糖和多元醇在内的几种相容性溶质的含量，以帮助维持渗透平衡。TCA循环中涉及的代谢物水平（包括柠檬酸，酮戊二酸，富马酸和苹果酸）在主根中降低了。iki草酸途径中的代谢产物如奎尼酸和sh草酸的含量显着降低。这项研究揭示了原始根对干旱和盐胁迫的综合代谢反应，并扩展了我们对玉米对非生物耐受性的根反应所涉及的机制的理解。苹果酸和苹果酸在根部被还原。iki草酸途径中的代谢产物如奎尼酸和sh草酸的含量显着降低。这项研究揭示了原始根对干旱和盐胁迫的综合代谢反应，并扩展了我们对玉米对非生物耐受性的根反应所涉及的机制的理解。苹果酸和苹果酸在根部被还原。iki草酸途径中的代谢物如奎尼酸和sh草酸的含量显着降低。这项研究揭示了原始根对干旱和盐胁迫的综合代谢反应，并扩展了我们对玉米对非生物耐受性的根反应所涉及的机制的理解。