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ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2020-12-11 , DOI: 10.1016/s2095-3119(20)63361-5
Chi REN , Cheng-li HOU , De-quan ZHANG , Xin LI , Xiong XIAO , Yu-qiang BAI

Phosphorylation post-translational modification plays an important role in postmortem muscle quality traits. Adenosine triphosphate (ATP) is an energy source and a key substrate of phosphorylation which provides the phosphatase groups to proteins in the presence of protein kinases. However, in postmortem muscle, the effects of ATP content on phosphorylation are poorly studied. The study investigated the effect of ATP on protein phosphorylation and degradation in postmortem ovine muscle. The ground muscle with/without additional ATP were treated/control groups and stored at 25 and 4°C, respectively. The ATP content led to different changes of pH value between the ATP-treated and control groups. The phosphorylation level of myofibrillar proteins was higher (P<0.05) in ATP-treated group compared to the control group at both temperatures, which suggested that ATP played a vital role in postmortem protein phosphorylation. A slower degradation rate of µ-calpain, desmin and troponin T was observed in the ATP-treated group which showed that there was a negative relationship between ATP level and the degradation of proteins. These observations clearly highlighted the role of ATP on the development of meat quality by regulating the phosphorylation and degradation of myofibrillar proteins in postmortem ovine muscle.



中文翻译:

ATP调节地面绵羊肌肉中肌原纤维蛋白的磷酸化和降解

磷酸化翻译后修饰在尸体肌肉质量特征中起重要作用。三磷酸腺苷(ATP)是一种能源,是磷酸化的关键底物,可在存在蛋白激酶的情况下为蛋白质提供磷酸酶基团。然而,在死后肌肉中,ATP含量对磷酸化的影响研究很少。该研究调查了ATP对死后绵羊肌肉中蛋白质磷酸化和降解的影响。将具有/不具有额外ATP的地面肌肉作为治疗/对照组,分别储存在25℃和4℃下。ATP含量导致ATP处理组和对照组之间的pH值发生不同的变化。在两个温度下,ATP处理组的肌原纤维蛋白的磷酸化水平均高于对照组(P <0.05),这表明ATP在死后蛋白质的磷酸化中起着至关重要的作用。在ATP处理组中,观察到的μ-钙蛋白酶,结蛋白和肌钙蛋白T的降解速度较慢,这表明ATP水平与蛋白质降解之间存在负相关关系。这些观察结果清楚地表明了ATP通过调节死后绵羊肌肉中肌原纤维蛋白的磷酸化和降解在肉质发展中的作用。

更新日期:2020-12-13
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