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Calsyntenin 1 mRNA expression sensitivity to ionizing radiation in human hepatocytes and carcinoma cells and blood cells of BALB/c mice
Journal of Radiation Research and Applied Sciences ( IF 1.7 ) Pub Date : 2020-12-11
Eon-Seok Lee, Won-Tae Kim, Ga-Young Park, Manwoo Lee, Tae Gen Son

ABSTRACT

Whether calsyntenin 1 (CLSTN1) and serine hydroxymethyltransferase 2 (SHMT2) are sensitive to low-dose ionizing radiation (LDIR) has not yet been defined. Therefore, here, we aimed to investigate changes in gene expression in hepatic cell lines and in blood cells and serum from irradiated mice. Levels of SHMT2 and CLSTN1 were decreased in cancer (HepG2) and normal (HH) hepatocytes exposed to various radiation doses. The mRNA expression of SHMT2 and CLSTN1 was altered in response to 0.5 or 4 Gy of radiation at 24 h post irradiation in vivo. However, plasma SHMT2 protein levels did not change at either radiation dose relative to those in the non-irradiated group. Plasma CLSTN1 protein levels notably decreased in response to 4 Gy of radiation but were not altered in response to 0.5 Gy of radiation. Thus, our findings suggest that the mRNA and protein levels of CLSTN1 might be useful biomarkers for radiation exposure. Further studies are needed to characterize the molecular regulation and functional roles of SHMT2 and CLSTN1 in response to radiation.



中文翻译:

Calsyntenin 1 mRNA表达对BALB / c小鼠肝细胞和癌细胞及血细胞电离辐射的敏感性

摘要

尚未确定Calsyntenin 1(CLSTN1)和丝氨酸羟甲基转移酶2(SHMT2)是否对低剂量电离辐射(LDIR)敏感。因此,在这里,我们旨在研究辐射小鼠的肝细胞系以及血细胞和血清中基因表达的变化。在暴露于各种辐射剂量的癌症(HepG2)和正常(HH)肝细胞中,SHMT2和CLSTN1的水平降低。在体内照射后24 h ,SHMT2CLSTN1的mRNA表达响应于0.5或4 Gy的辐射而改变。但是,相对于未辐照组,血浆SHMT2蛋白水平在两种辐照剂量下均没有变化。血浆CLSTN1蛋白水平响应4 Gy辐射显着降低,但响应0.5 Gy辐射则未改变。因此,我们的发现表明CLSTN1的mRNA和蛋白水平可能是辐射暴露的有用生物标志物。需要进一步的研究来表征SHMT2和CLSTN1响应辐射的分子调控和功能作用。

更新日期:2020-12-11
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