This paper proposes a colorimetric aptasensor for the detection of testosterone (TES) in environmental water, using TES-specific aptamer (apT5) as a sensing probe, gold nanoparticles (AuNPs) as indicator, and hexadecyltrimethylammonium bromide (CTAB) as inducer, respectively. Based on competition between TES and CTAB for apT5, the aptamer can form an aptamer–TES complex, leaving CTAB free to aggregate AuNPs in the presence of TES. Dispersed and aggregated AuNPs have different absorption wavelengths and the signal of absorption intensity is associated with the concentration of TES, so TES can be detected quantitatively based on the signal absorption intensity. This sensitive aptasensor for TES detection has a wide linear range (R = 0.998) from 1.91–800 nM and a limit of detection (LOD) of 1.91 nM. In addition, this aptasensor has high selectivity over some interferents. The method detects TES in tap water samples with recoveries in the range of 98.9–102.6 % (RSD ≤ 7.35 %). This biosensor presents a good and potential application to rapidly detect TES in actual environmental water samples.

本文提出了一种比色适体传感器，用于检测环境水中的睾丸激素（TES），分别使用TES特异性适体（apT5）作为传感探针，金纳米颗粒（AuNPs）作为指示剂和十六烷基三甲基溴化铵（CTAB）作为诱导剂。根据TES和CTAB之间针对apT5的竞争，适体可以形成aptamer-TES复合物，而CTAB在TES存在下可自由聚集AuNP。分散和聚集的AuNPs具有不同的吸收波长，并且吸收强度的信号与TES的浓度有关，因此可以根据信号吸收强度定量检测TES。这种用于TES检测的灵敏适体传感器具有宽线性范围（R = 0.998）从1.91–800 nM，检测限（LOD）为1.91 nM。另外，该适体传感器对某些干扰物具有高选择性。该方法检测自来水样品中的TES，回收率在98.9–102.6％（RSD≤7.35％）范围内。这种生物传感器为快速检测实际环境水样中的TES提供了很好的潜在应用。