Late embryogenesis-abundant (LEA) proteins are the products of an important gene family in plants that play vital roles in regulating growth and development as well as a variety of stress responses. In our study, 67 members of LEA (BdLEA) were identified in the genome of Brachypodium distachyon L. Analyses of gene structure, evolutionary relationships and protein motifs showed that the BdLEAs belonged to six subfamilies. Analyses of chromosomal locations and duplication events revealed that the 67 BdLEAs were distributed over all five chromosomes and 26 BdLEAs were identified as products of duplication events. Gene Ontology (GO) annotation results suggested that nearly 60% of BdLEAs could be involved in stress response. Furthermore, transcriptomic analysis showed that the BdLEAs were differentially expressed in nine organs and responded to low stringency of exogenous phytohormones. Subsequently, 18 BdLEAs from six subfamilies were randomly selected for quantitative real-time PCR (qRT-PCR) analysis, which showed that they were mainly expressed in the spikelets and they may preferentially respond to salt, drought and abscisic acid (ABA) stress. This study is the first to report the characteristics of the BdLEA family, providing valuable information for understanding the evolution of LEAs in the model plant B. distachyon and supporting future functional research on these proteins.

晚期胚胎发生（LEA）蛋白是植物中重要基因家族的产物，在调节生长和发育以及各种胁迫反应中起着至关重要的作用。在我们的研究中，LEA的67个成员国（BdLEA）的基因组进行鉴定穗短柄草基因结构分析L.，进化关系和蛋白质的图案表明，BdLEA小号属于六个亚科。染色体位置和复制事件的分析表明，67个BdLEA分布在所有五个染色体上，而26个BdLEA被鉴定为复制事件的产物。基因本体论（GO）注释结果表明，近60％的BdLEA可能与压力反应有关。此外，转录组分析显示，BdLEA在9个器官中差异表达，并对低度的外源植物激素作出反应。随后，随机选择了六个亚科的18个BdLEA用于实时定量PCR（qRT-PCR）分析，结果表明它们主要在小穗中表达，并且可能优先响应盐，干旱和脱落酸（ABA）胁迫。 。这项研究是第一个报告BdLEA家族特征的研究，为了解模型植物B. distachyon中LEA的进化提供了有价值的信息，并支持了对这些蛋白质的未来功能研究。