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Evaluation of sperm morphometry of rabbits (Oryctolagus cuniculus f. domesticus)
Reproduction in Domestic Animals ( IF 1.7 ) Pub Date : 2020-12-11 , DOI: 10.1111/rda.13876
Olga Szeleszczuk 1 , Katarzyna Andraszek 2 , Dorota Banaszewska 2 , Piotr Niedbała 1 , Marta Kuchta-Gładysz 1 , Anna Grzesiakowska 1
Affiliation  

Sperm morphology and morphometry are considered parameters in fertility diagnosis. They are especially important in the case of species for which there is no standard with respect to morphometric sperm parameters. It is then crucial to apply the staining technique that has the least influence on the sperm structure and provides the most detailed image, so as to enable measurements. The aim of the research was to assess the morphometric parameters of rabbit sperm using silver nitrate staining. The staining process revealed a detailed image of the spermatozoon head and tail, thus enabling precise measurements. From these basic morphometric parameters, four additional shape indices characterizing the sperm head were calculated: ellipticity, elongation, roughness and regularity. These parameters more precisely characterize the shape of the sperm head. Silver nitrate staining can be used as an independent technique in assessment of sperm structure or to supplement routine diagnostics.

中文翻译:

兔精子形态计量学评价(Oryctolagus cuniculus f.domesticus)

精子形态和形态测量被认为是生育诊断的参数。对于精子形态参数没有标准的物种,它们尤其重要。因此,至关重要的是应用对精子结构影响最小并提供最详细图像的染色技术,以便进行测量。该研究的目的是使用硝酸银染色评估兔精子的形态测量参数。染色过程揭示了精子头部和尾部的详细图像,从而能够进行精确测量。根据这些基本的形态测量参数,计算了表征精子头部的四个附加形状指数:椭圆度、伸长率、粗糙度和规则性。这些参数更准确地表征了精子头部的形状。
更新日期:2020-12-11
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