Aims

Field pea (Pisum sativum L.) is an important rotation crop in agriculture on the Canadian prairies. Pea root rot is caused by a complex of soilborne pathogens, and can have a tremendous impact on reducing yields and removing fields from pea production. The objectives of this study were to: determine whether characteristics of the fungal and bacterial microbiome are associated with diseased or healthy sites within pea fields, and assess whether abiotic soil properties differed between these diseased or healthy sites.

Methods

Soil was collected from healthy and diseased sites within 5 fields across Alberta. Bacterial and fungal operational taxonomy units were determined using barcoded Illumina MiSeq of the 16S rRNA and internal transcribed spacer 1 (ITS) genes, respectively. Soil enzyme activities and soil physicochemical properties were also measured.

Results

Principal coordinate analysis showed that health status was an important determinant of bacterial communities in two out of five fields and fungal communities in all fields. Microbial communities also differed between locations. However, there was no clear relationship between specific fungal or bacterial families, enzyme activities or soil properties with root rot status.

Conclusions

Although root rot severity and fungal community composition varied between healthy and diseased sites within a field, there was no consistent correlation of disease severity with biotic or abiotic soil properties across locations.

中文翻译：

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土壤的细菌和真菌群落，而不是理化性质，取决于豌豆的根腐状态

目的

豌豆（Pisum sativum L.）是加拿大大草原农业上重要的轮作作物。豌豆根腐病是由多种土壤传播的病原体引起的，可能对降低产量和从豌豆生产中除掉田地产生巨大影响。这项研究的目的是：确定真菌和细菌微生物组的特征是否与豌豆田中的患病或健康场所有关，并评估这些患病或健康场所之间非生物土壤特性是否有所不同。

方法

从艾伯塔省5个田地的健康和患病地点采集土壤。分别使用16S rRNA的带条形码的Illumina MiSeq和内部转录的spacer 1（ITS）基因确定细菌和真菌的操作分类单位。还测量了土壤酶活性和土壤理化性质。

结果

主坐标分析表明，健康状况是五个领域中两个领域和所有领域中真菌群落的重要决定因素。地点之间的微生物群落也不同。但是，具体的真菌或细菌家族，酶活性或土壤特性与根腐病之间没有明确的关系。

结论

尽管田间健康和患病部位的根腐病严重程度和真菌群落组成各不相同，但疾病严重程度与各地的生物或非生物土壤特性之间没有一致的相关性。