Abstract

Laccase, as an important biocatalyst, has aroused wide public interests in the field of environment and catalysis. However, the application of laccase in large-scale is still limited due to its high solubility and poor stability. In order to improve the reusability of laccase, immobilization with functional materials has attracted increasing attention. Metal-organic frameworks (MOFs) stand out as a promising carrier for its high adsorption capacity and flexible structure. The present paper reviews the current status and remaining challenges in the immobilization of laccase with MOFs. Furthermore, three methods are proposed to further improve the immobilization of laccase with MOFs, including co-immobilization of laccase and mediator, co-immobilization of laccase and other enzymes, and coupling of biocatalysis with photochemical reactions. This work, as the first review article on the immobilization of laccase with MOFs, will provide a navigation map for the subsequent selection and design of MOFs for more targeted immobilization of laccase.

摘要

漆酶作为一种重要的生物催化剂，在环境和催化领域引起了广泛的关注。然而，漆酶由于溶解度高、稳定性差等问题，其大规模应用仍受到限制。为了提高漆酶的可重复使用性，功能材料的固定化引起了越来越多的关注。金属有机框架（MOFs）因其高吸附能力和灵活的结构而成为一种很有前途的载体。本文回顾了用 MOF 固定漆酶的现状和仍然存在的挑战。此外，提出了三种方法来进一步改善漆酶与 MOFs 的固定化，包括漆酶和介质的共固定化、漆酶和其他酶的共固定化以及生物催化与光化学反应的耦合。