Abstract The present study profiled various chemical constituents from the ethyl acetate fraction of Kigelia africana fruit extract in a comparative manner using modern chromatographic methods coupled with mass spectrometric techniques. K. africana plant has a wide usage in folklore medicine for numerous ailments with the ethyl acetate fraction having high antifungal and anti-plasmodial activities. Dried fruits were extracted with ethanol and partitioned using ethyl acetate. The fraction was analyzed using GC-MS, GC-TOF-MS and UHPLC-TOF-MS/MS. Fifteen compounds consisting of 2-methoxy-4-vinylphenol, phenol-2,4-bis(1,1-dimethylethyl), - and 9,12,15-octadecatrienoic acid ethyl ester, (Z, Z, Z) – were obtained from GC-MS while 441 metabolites ranging from flavonoids, cardiac glycosides, and polyphenols were profiled from GC-TOF-MS whereas 235 metabolites were fingerprinted from UHPLC-TOF-MS/MS analysis by measuring m/z values and analyzing MS fragmentation pattern. From the identified phytochemicals through ultra-high-pressure liquid chromatography tandem mass spectrometer (UHPLC-TOF-MS/MS), 14 of them were confirmed and these include gemfibrozil, trimetazidine, and leucomalachite green.

中文翻译：

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Kigelia Africana 果实提取物乙酸乙酯馏分的代谢物指纹图谱：使用 GC-MS、GC-TOF-MS 和 UHPLC-TOF-MS/MS 比较化学成分。

摘要 本研究使用现代色谱方法与质谱技术相结合，以比较方式分析了非洲非洲菊果实提取物乙酸乙酯级分中的各种化学成分。K. Africana 植物在民间传说医学中广泛用于治疗多种疾病，其乙酸乙酯成分具有高抗真菌和抗疟原虫活性。干果用乙醇提取并用乙酸乙酯分配。使用 GC-MS、GC-TOF-MS 和 UHPLC-TOF-MS/MS 分析馏分。获得了由 2-甲氧基-4-乙烯基苯酚、苯酚-2,4-双（1,1-二甲基乙基）、-和 9,12,15-十八碳三烯酸乙酯（Z、Z、Z）组成的 15 种化合物来自 GC-MS 而 441 种代谢物，包括类黄酮、强心苷、GC-TOF-MS 对多酚进行了分析，而 UHPLC-TOF-MS/MS 通过测量 m/z 值和分析 MS 碎裂模式对 235 种代谢物进行了指纹图谱分析。从通过超高压液相色谱串联质谱仪 (UHPLC-TOF-MS/MS) 鉴定的植物化学物质中，确认了 14 种植物化学物质，其中包括吉非贝齐、曲美他嗪和隐色孔雀石绿。