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Microbial Control of the Emerald Ash Borer (Coleoptera: Buprestidae) Using Beauveria bassiana (Hypocreales: Cordycipitaceae) by the Means of an Autodissemination Device
Journal of Economic Entomology ( IF 2.2 ) Pub Date : 2020-10-22 , DOI: 10.1093/jee/toaa226
Narin Srei 1 , Claude Guertin 1 , Robert Lavallée 2 , Marie-Ève Lajoie 3 , Christian Brousseau 3 , Réjean Bergevin 3 , Fredric Miller 4 , Kendra McMillin 5 , Richard Trudel 6
Affiliation  

The FraxiProtec, an autodissemination device loaded with the fungus Beauveria bassiana isolate CFL-A, was tested in the field to evaluate its potential to infect emerald ash borer adults and reduce their populations. During the 2-yr experimental period, the dispersion of B. bassiana-infected adults was also documented to assess the dissemination capacity of the biocontrol agent beyond the treated areas. The mean percentage of infected emerald ash borer in 2017 and 2018 in 15 treated sites was 43.3 ± 2.9% and 39.7 ± 3.9%, respectively, and no significant variation was observed over the tested years. Furthermore, a 40% significant reduction of the mean emerald ash borer population growth per tree in treated sites was recorded when compared to the control sites. Emerald ash borer infected with B. bassiana isolate CFL-A were collected from baited sticky traps in the sentinel stations in the area surrounding the FraxiProtec-treated sites. Even at a distance of 125 m, an average of 9.4 ± 2.6% infected emerald ash borer were collected. Finally, exploratory analyzes were carried out on parameters such as the area to be treated, ash tree density, and FraxiProtec density to document potential relationships, which could be used in the determination of a prescription.

中文翻译:

通过自动传播装置使用白僵菌(Hypocreales:Cordycipitaceae)对翡翠灰蛀虫(鞘翅目:Buprestidae)的微生物控制

FraxiProtec 是一种装载有真菌白僵菌分离株 CFL-A 的自动传播装置,在现场进行了测试,以评估其感染翡翠灰螟成虫并减少其种群数量的潜力。在 2 年的实验期间,还记录了 B. bassiana 感染的成虫的分散情况,以评估生物防治剂在处理区域之外的传播能力。2017 年和 2018 年在 15 个处理地点感染的祖母绿灰蛀虫的平均百分比分别为 43.3 ± 2.9% 和 39.7 ± 3.9%,并且在测试年份中没有观察到显着变化。此外,与对照地点相比,处理地点每棵树的平均翡翠灰螟种群增长显着减少了 40%。感染了 B. 从 FraxiProtec 处理地点周围区域的哨兵站中的诱饵粘性陷阱中收集了 Basiana 分离株 CFL-A。即使在 125 m 的距离内,平均也能收集到 9.4 ± 2.6% 的感染祖母绿灰螟。最后,对待处理的面积、白蜡树密度和 FraxiProtec 密度等参数进行探索性分析,以记录可用于确定处方的潜在关系。
更新日期:2020-10-22
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