Abstract

Exposure to crude acetylene can occur in occupational settings. This study assessed the modulatory activities of selected polyphenols on the hematotoxic, cardiotoxic, and hepatotoxic effects of crude acetylene. Wistar rats were exposed to 58 000 ppm crude acetylene for 10 min at 12 h intervals for 30 days. Some exposed groups were treated with 50 mg/kg rutin, quercetin, gallic acid, or tannic acid. Indices of hematological disorder, oxidative stress, and cardiovascular and hepatocellular injuries were evaluated in animals. The results showed a decrease in the levels of hematological indices in crude acetylene-exposed animals except for white blood cell count which was increased. Decreased activity/level of reduced glutathione, superoxide dismutase and ferric reducing antioxidant power with increased lipid peroxidation was observed in animals exposed to crude acetylene. Activities of transaminases, γ-glutamyl transpeptidase, and level of bilirubin were increased while the plasma albumin level was decreased. Dyslipidemia, increased activities of lactate dehydrogenase and creatine kinase-MB, and severe histopathological damage to hepatic and cardiac tissues were also observed in animals exposed to the gas. These deleterious hematological, biochemical, and histopathological changes were ameliorated in crude acetylene–toxified rats treated with the polyphenols. Tannic acid exhibited better activity than gallic acid while quercetin showed a superior activity to rutin. The results indicate that exposure to crude acetylene can lead to blood, heart, and liver-related diseases and dietary polyphenols could provide protective benefits.

摘要

在职业环境中可能会接触到粗乙炔。这项研究评估了所选多酚对粗乙炔的血液毒性，心脏毒性和肝毒性作用的调节活性。将Wistar大鼠以12 h的间隔暴露于58 000 ppm粗乙炔中10分钟，持续30天。一些暴露的人群接受了50 mg / kg芦丁，槲皮素，没食子酸或单宁酸的治疗。在动物中评估了血液系统疾病，氧化应激以及心血管和肝细胞损伤的指标。结果表明，暴露于粗乙炔的动物血液学指标水平有所下降，但白细胞计数有所增加。降低的活性/还原型谷胱甘肽的水平，在暴露于粗乙炔的动物中观察到超氧化物歧化酶和三氧化二铁降低了抗氧化能力，并增加了脂质过氧化作用。转氨酶活性，γ-谷氨酰转肽酶活性和胆红素水平升高，而血浆白蛋白水平降低。在暴露于该气体的动物中还观察到血脂异常，乳酸脱氢酶和肌酸激酶-MB活性增加以及对肝和心脏组织的严重组织病理学损害。这些有害的血液学，生化和组织病理学变化在用多酚处理的粗乙炔中毒的大鼠中得到改善。单宁酸显示出比没食子酸更好的活性，而槲皮素显示出比芦丁更高的活性。结果表明，暴露于粗乙炔会导致血液，心脏，