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NMR Metabolic Profiling of Sargassum Species Under Different Stabilization/Extraction Processes
Journal of Phycology ( IF 2.9 ) Pub Date : 2020-12-09 , DOI: 10.1111/jpy.13117
Gloria Ivonne Hernández‐Bolio 1 , Adrián Fagundo‐Mollineda 1 , Edgar Emmanuel Caamal‐Fuentes 1 , Daniel Robledo 1 , Yolanda Freile‐Pelegrin 1 , Emanuel Hernández‐Núñez 1, 2
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The genus Sargassum is well represented by benthic and pelagic species, some of which form massive aggregates that can travel long distances due to the force of the ocean currents. Although they constitute an essential habitat for fish and invertebrate species, large accumulations of Sargassum in coastal areas generate several economic, environmental, and health impacts. It is important to recognize the species forming these aggregates, and identify the metabolites they produce, allowing for its exploitation, and therefore, better management practices. NMR metabolic profiling is a technique that can discriminate samples while detecting their unique or differential chemical features, and has been successfully used in the study and classification of several algal species. The present investigation studied the metabolic profiling of Sargassum species found on strandings at Puerto Morelos (Quintana Roo) east coast of the Mexican Caribbean. PCA of the 1H‐NMR profiles corresponding to S. natans, S. natans (morphotype VIII), S. fluitans, and a benthic Sargassum buxifolium allowed the discrimination of samples amongst them. Furthermore, discrimination between the two forms of S. natans was also possible. The PCA loading plot revealed that glutamine and glutamate have the highest influence in the clustering of the benthic Sargassum, while a high abundance of lactate, Myo‐inositol, and trimethylamine is a unique feature from the S. natans morphotype VIII. Additional PLS‐DA models showed that a heat‐drying process improved the extraction of metabolites. Maceration and microwave‐assisted extraction with water‐ethanol led to similar profiles and thus any of them could be used in future investigations.

中文翻译:

不同稳定/提取过程下马尾藻物种的NMR代谢谱分析

鼠尾藻深受深海和远洋物种,其中一些形成块状集合体,可以长途跋涉,由于洋流的力表示。尽管它们构成了鱼类和无脊椎动物物种的重要栖息地,但大量的马尾藻在沿海地区产生一些经济,环境和健康影响。重要的是要识别形成这些聚集体的物种,并确定它们产生的代谢产物,以便对其进行开发,从而改善管理方法。NMR代谢谱分析是一种可以在检测样品独特或差异化学特征的同时对其进行区分的技术,并且已成功用于几种藻类的研究和分类。本研究研究了在墨西哥加勒比海东海岸的从莫雷洛斯港(金塔纳罗奥州)搁浅的海藻物种的代谢谱。1 H-NMR谱的PCA对应于S. natansS。natans(VIII型),S. fluitans和底栖的Sargassum buxifolium可以区分其中的样品。此外,两种形式的纳丹链球菌之间的区别也是可能的。PCA加载图显示,谷氨酰胺和谷氨酸对底栖海藻的聚集具有最大的影响,而乳酸,肌醇和三甲胺的高丰度是纳丹链球菌VIII型的独特特征。附加的PLS-DA模型显示,热干燥过程改善了代谢物的提取。用水浸软化和微波辅助萃取得到的谱图相似,​​因此它们中的任何一种都可用于将来的研究。
更新日期:2020-12-09
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