Ngari virus is a mosquito-borne virus belonging to the genus Orthobunyavirus (Peribunyaviridae family). This virus is pathogenic to humans and causes severe illness. Ngari virus is present in several African countries, including Madagascar. Here, we report the detection of Ngari virus in ixodid ticks collected from cows in Guinea.

A tick survey was conducted in March-November of 2018 in six regions of Guinea. The sample comprised 710 pools, with a total of 2067 ticks belonging to five species collected from 197 cows. At the initial stage, we screened a subsample of tick pools of vector-borne viruses with a multiplex genus-specific primer panel. In the second stage of the study, we narrowed the search and screened all the samples by qPCR for the detection of Ngari virus. All positive samples were sequenced with primers flanking Ngari virus-specific fragments on the S and M segments.

We found Ngari virus in 12 pools that were formed from engorged ticks collected from livestock in three villages of the Kindia and Kankan regions. Sequencing of the S and M segments confirmed that the detected viruses belong to Ngari virus, and the viruses were most similar to the strain Adrar, which was isolated in Mauritania. We detected viral RNA in ticks of the following species: Amblyomma variegatum, Rhipicephalus geigyi, and Rh. (Boophilus) spp. There is no evidence that ixodid ticks are competent vectors of the Ngari virus. Most likely, the ticks obtained the virus through blood from an infected host. The study of engorged ticks can be recommended as a simpler approach for the wide screening of the Ngari virus and subsequent testing of cattle and mosquitos in those locations where the PCR-positive ticks were collected.

Ngari病毒是一种由蚊子传播的病毒，属于Orbunya病毒属（Peribunyaviridae家族）。该病毒对人类有致病性，并导致严重疾病。Ngari病毒存在于包括马达加斯加在内的几个非洲国家。在这里，我们报告了在几内亚的母牛身上收集的ixodid tick中检测到Ngari病毒的情况。

2018年3月至11月在几内亚的六个地区进行了刻度调查。样本包括710个池，共有2067个s，属于从197头牛中收集的五个物种。在初始阶段，我们使用多重属特异性引物筛选了载体传播的病毒滴答池的子样本。在研究的第二阶段，我们缩小了搜索范围，并通过qPCR筛选了所有样本以检测Ngari病毒。所有阳性样品均在S和M片段上使用Ngari病毒特异性片段侧翼的引物进行测序。

我们在Kindia和Kankan地区三个村庄的牲畜采集的饱满的formed形成的12个池中发现了Ngari病毒。S和M区段的测序证实了所检测到的病毒属于Ngari病毒，并且这些病毒与在毛里塔尼亚分离的Adrar毒株最相似。我们在以下物种的壁虱中检测到病毒RNA：变异羊草，Rhipicephalus geigyi和Rh。（Boophilus）spp。没有证据表明ixodid s是Ngari病毒的有效载体。likely虫很可能是从受感染宿主的血液中感染病毒的。可以建议对虫进行研究，这是更简单的方法，可广泛筛选Ngari病毒，并随后在收集PCR阳性tick虫的地方进行牛和蚊子的检测。