Low strength and rapid biodegradability of acellular dermal matrix (ADM) restrict its wider clinical application as a rapid cell delivery platform in situ for management of burn wounds. Herein, the extracted ADM was modified by a dual cross-linking approach with ionic crosslinking using chitosan and covalent cross-linking using an iodine-modified 2,5-dihydro-2,5-dimethoxy-furan cross-linker, termed as CsADM-Cl. In addition, inherent growth factors and cytokines were found to be preserved in CsADM-Cl, irrespective of ionic/covalent crosslinking. CsADM-Cl demonstrated improvement in post crosslinking stiffness with a decreased biodegradation rate. This hybrid crosslinked hydrogel supported adhesion, proliferation, and migration of human foreskin-derived fibroblasts and keratinocytes. Also, the angiogenic potential of CsADM-Cl was manifested by chick chorioallantoic membrane assay. CsADM-Cl showed excellent antibacterial activity against Escherichia coli and Staphylococcus aureus. Moreover, CsADM-Cl treated full thickness burn wounds and demonstrated rapid healing marked with superior angiogenesis, well-defined dermal–epidermal junctions, mature basket weave collagen deposition, and development of more pronounced secondary appendages. Altogether, the bioactive CsADM-Cl hydrogel established significant clinical potential to support wound healing as an apt injectable antibacterial matrix to encounter unmet challenges concerning critical burn wounds.

中文翻译：

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烧伤创面的双功能性可注射混合细胞外基质水凝胶

无细胞真皮基质（ADM）的低强度和快速生物降解性限制了其作为快速原位细胞递送平台的广泛临床应用用于烧伤伤口的处理。本文中，提取的ADM通过双交联方法进行改性，其中使用壳聚糖进行离子交联，使用碘改性的2,5-二氢-2,5-二甲氧基呋喃交联剂（称为CsADM- Cl。另外，发现固有生长因子和细胞因子被保留在CsADM-Cl中，而与离子/共价交联无关。CsADM-Cl证明了后交联刚度的提高，生物降解率降低。这种杂交交联的水凝胶支持人包皮来源的成纤维细胞和角质形成细胞的粘附，增殖和迁移。而且，CsADM-Cl的血管生成潜力通过鸡绒膜尿囊膜测定法得以证实。CsADM-Cl对大肠杆菌和大肠杆菌具有出色的抗菌活性。金黄色葡萄球菌。此外，CsADM-Cl处理了全层烧伤创面，并显示出快速愈合的特点，具有优越的血管生成，明确的表皮-表皮交界处，成熟的篮式胶原沉积和更明显的继发附件的形成。总之，具有生物活性的CsADM-Cl水凝胶具有很强的临床潜力，可作为一种可注射的抗菌基质来支持伤口愈合，以应对有关严重烧伤创面的未解决挑战。