Abstract

Etlingera sayapensis belongs to Zingiberaceae family. This research tried to evaluate the chemical composition and bioactivity of E. sayapensis fruits essential oil (ESFO) and its methanolic extract (ES-FM). The essential oil was obtained using a Clevenger-type apparatus. ESFO composition was identified by chromatographic-based methods (GC/FID and GC/MS). The evaluation of the bioactivity of ESFO and EFSM including antioxidant, antimicrobial and antiproliferative activity was carried out using various assays. Linalool formate (41.2 %), α-terpineol (17.9 %), δ-elemene (14.4 %) and octyl formate (12.8 %) were the most abundant compounds in ESFO. The plant extract exhibited potent antioxidant activity, as well. ESFM scavenged DPPH with an IC₅₀ of 6.11±0.42 μg/mL, bleached β-carotene with 95.61±0.92 percentage, a chelated ferrous ion with IC₅₀ of 139.75±6.3 μg/mL and reduced ferric ion with 67.72±0.93 mg TrE/g. the total phenolic content of ESFM was 57.13±0.72 mg GAE/g. Although ESFO showed a weaker activity compared to EFSM. On the other hand, ESFO revealed antimicrobial activity as well as ESFM in disc diffusion and minimum inhibition concentration assays. They prevented the growth of 13 and 10 out of 18 selected microorganisms respectively. In MTT assay, ESFM exerted cytotoxic effects on human prostate (IC₅₀= 26.52±2.15 μg/mL), colon (IC₅₀= 49.60±1.60 μg/mL) and breast (IC₅₀ = 69.82±1.98 μg/mL) cancer cells, EFSO also displayed effects on the cancer cells but less than those of EFSM. In conclusion, our study has illustrated that the fruits of E. sayapensis can be considered as a rich source of secondary metabolites with real benefits.

摘要

Etlingera sayapensis属于姜科。这项研究试图评估大肠沙门氏菌果实精油（ESFO）及其甲醇提取物（ES-FM）的化学成分和生物活性。使用Clevenger型设备获得精油。通过基于色谱的方法（GC / FID和GC / MS）鉴定ESFO组成。使用各种测定法对ESFO和EFSM的生物活性进行了评估，包括抗氧化剂，抗微生物剂和抗增殖活性。ESFO中含量最高的化合物是芳樟醇（41.2％），α-松油醇（17.9％），δ-榄香烯（14.4％）和甲酸辛酯（12.8％）。植物提取物也表现出有效的抗氧化活性。具有IC _50的ESFM清除DPPH样品中的有机物含量为6.11±0.42μg/ mL，漂白的β-胡萝卜素含量为95.61±0.92％，螯合的亚铁离子的IC ₅₀为139.75±6.3μg/ mL，还原的铁离子含量为67.72±0.93 mg TrE / g。ESFM的总酚含量为57.13±0.72 mg GAE / g。尽管与EFSM相比，ESFO的活动较弱。另一方面，ESFO在椎间盘扩散和最小抑制浓度试验中显示了抗菌活性以及ESFM。他们分别阻止了18种选定微生物中的13种和10种的生长。在MTT分析中，ESFM对人前列腺（IC ₅₀ = 26.52± 2.15μg / mL），结肠（IC ₅₀ = 49.60±1.60μg/ mL）和乳腺（IC ₅₀）具有细胞毒性作用EFSO（= 69.82±1.98μg/ mL）癌细胞也显示出对癌细胞的作用，但小于EFSM。总之，我们的研究表明，大肠沙门氏菌的果实可以被认为是次生代谢产物的丰富来源，具有真正的益处。