Abstract

Essential oil (EO) of Volkamer lemon can be used in food, pharmaceutical and perfumery products. Nicotinic acid (NA) has a physiological role in EO biosynthesis. This study aimed to evaluate the EO isolated from leaves, peels and flowers of Volkamer lemon in response to NA treatments. Volkamer lemon trees were subjected to different doses of NA (0, 20 and 40 mg/l). The EO of leaves, peels and flowers were isolated by hydrodistillation method (HD), then, analyzed by GC and GC/MS. Data were statistically analyzed using ANOVA. The greatest amounts of leaf, peel and flower EO were recorded with NA at 20 mg/l. Sabinene and limonene were the major components of leaf EO; while limonene and γ-terpinene were identified as main components of peel EO; limonene, nerol, linalool and sabinene were the main components of flower EO. NA at 40 mg/l resulted in the maximum values of all major components. The characterized components of leaf and peel EO belonged to four chemical classes such as monoterpene hydrocarbons (MH), oxygenated monoterpenes (OM), sesquiterpene hydrocarbons (SH) and oxygenated sesquiterpenes (OS); while the identified components of flower EO belonged to five chemical classes such as MH, OM, SH, OS and diterpene hydrocarbons (DH). Different changes were found in various chemical classes due to NA doses.

摘要

Volkamer柠檬的精油（EO）可用于食品，药品和香料产品。烟酸（NA）在EO生物合成中具有生理作用。这项研究旨在评估从NA处理中从Volkamer柠檬的叶子，果皮和花朵中分离得到的EO。Volkamer柠檬树受到不同剂量的NA（0、20和40 mg / l）。通过加氢蒸馏法（HD）分离叶子，果皮和花朵的EO，然后通过GC和GC / MS分析。使用ANOVA对数据进行统计分析。以20 mg / l的NA记录到最大的叶片，果皮和花朵EO含量。bin烯和柠檬烯是叶片EO的主要成分。柠檬烯和γ-萜品烯被认为是果皮EO的主要成分。柠檬烯，橙花油，芳樟醇和sa烯为花EO的主要成分。40 mg / l的NA导致所有主要成分的最大值。叶和果皮EO的特征成分属于四个化学类别，例如单萜碳氢化合物（MH），氧化单萜（OM），倍半萜碳氢化合物（SH）和氧化倍半萜烯（OS）；花中EO的成分分为五类，如MH，OM，SH，OS和二萜碳氢化合物（DH）。由于NA剂量，在各种化学类别中发现了不同的变化。