Abstract

Amomum velutinum X.E.Ye, Škornièk. & N.H.Xia (local name: Sa nhân lông hung) has been used in ethnomedicine for the amelioration of fever, rheumatism and microbial infections among others. In this study, the chemical constituents and antimicrobial activity of essential oils from the leaf, pseudo-stem and rhizome of A. velutinum were described. The study includes hydrodistillation of essential oils from the plant parts, instrumental analysis of the essential oils by gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC/MS) and the determination of the antimicrobial activity of the essential oils by the microdilution broth susceptibility assay. The essential oils were obtained in yields of 0.18 %, 0.11 and 0.21 % (v/w,±0.01) respectively for the leaf, pseudo-stem and rhizomes. β-Pinene (43.5 %-46.3 %) and α-pinene (5.2 %-8.1 %) were the main constituents of the essential oils. The leaf oil contained a significant quantity of myrtenyl acetate (10.5 %) and thymol (6.8 %), while β-caryophyllene (14.0 %), cis-β-elemene (9.8 %) and α-pinene (5.2 %) were found in the pseudo-stem oil in sizeable amount. The essential oils inhibited the growth of gram-positive bacteria namely Enterococcus faecalis ATCC299212 with minimum inhibitory concentration value (MIC) of 3.98-25. 56 μg/mL), Staphylococcus aureus ATCC25923 (MIC 32.87-100.56 μg/mL), Bacillus cereus ATCC14579 (MIC19. 78-20.45 μg/mL) and a strain of yeast, Candida albicans ATCC10231 (MIC 20.56-55.67 μg/mL). This was the first report on the chemical compositions and antimicrobial activity of essential oils from A. velutinum. The results are an indication of the potential of A. velutinum essential oils as a source of antimicrobial agents.

摘要

伏特加（Amomum velutinum） XEYe，Škornièk。＆NHXia（当地名称：Sanhânlônghung）已被用于人种医学，以改善发烧，风湿病和微生物感染等。在这项研究中，从A. velutinum的叶子，假茎和根茎中提取的精油的化学成分和抗菌活性被描述。该研究包括对植物部位精油进行加氢蒸馏，通过气相色谱仪（GC）和气相色谱仪与质谱（GC / MS）进行仪器分析以及通过微稀释法测定精油的抗菌活性。肉汤药敏试验。叶，假茎和根茎的精油分别以0.18％，0.11和0.21％（v / w，±0.01）的产率获得。β-P烯（43.5％-46.3％）和α-pine烯（5.2％-8.1％）是精油的主要成分。叶油中含有大量的乙酸烯丙酯（10.5％）和百里酚（6.8％），而β-石竹烯（14.0％），顺式在假茎油中发现大量的-β-榄香烯（9.8％）和α-pine烯（5.2％）。精油抑制革兰氏阳性细菌即粪肠球菌ATCC299212的生长，其最低抑菌浓度值（MIC）为3.98-25。56μg / mL），金黄色葡萄球菌ATCC25923（MIC 32.87-100.56μg/ mL），蜡状芽孢杆菌ATCC14579（MIC19。78-20.45μg/ mL）和一株酵母，白色念珠菌ATCC10231（MIC 20.56-55.67μg/ mL） 。这是关于A.velutinum精油化学成分和抗菌活性的首次报道。结果表明，A。velutinum精油有可能成为抗菌剂的来源。