Triacylglycerols (TAGs) can be modified to increase the absorption of fatty acids, prevent obesity, and treat fat malabsorption disorders and metabolic diseases. Medium–long–medium (MLM)-type TAGs, which contain medium-chain fatty acids in the sn-1 and sn-3 positions of the glycerol backbone and a long-chain fatty acid in the sn-2 position, show particularly interesting nutritional characteristics. This study aimed to synthesize MLM-type TAGs by enzymatic acidolysis of grape seed oil with medium-chain capric acid (C10:0) in associated packed bed reactors. The reaction was carried out during 120 H, at 45 °C, using lipase from Rhizomucor miehei (Lipozyme^® RM IM). The residence time distribution of reagents in the reactor was quantified to evaluate the reactor behavior and to diagnose the existence of preferential paths. The reaction progress was monitored by analyzing TAG composition and, at the steady state (after 48 H of reaction), the incorporation degree achieved a value of 39.91 ± 2.77%. To enhance the capric acid incorporation, an acidolysis reaction in associated packed bed reactors was performed. The results showed a good operational stability of the biocatalyst, revealing values of half-life 209.64 H, 235.63 H of packed bed and associated packed bed reactor, respectively, and a deactivation coefficient 0.0061 H⁻¹.

中文翻译：

---

在相关填充床反应器中从葡萄籽 (Vitis vinifera L.) 油中酶促合成结构化脂质

可以修饰三酰基甘油 (TAG) 以增加脂肪酸的吸收，预防肥胖，治疗脂肪吸收障碍和代谢疾病。中长中型 (MLM) 型 TAG，在甘油骨架的sn -1 和sn -3 位置含有中链脂肪酸，在sn -2 位置含有长链脂肪酸，表现出特别有趣营养特性。本研究旨在通过在相关填充床反应器中用中链癸酸 (C10:0) 对葡萄籽油进行酶酸解来合成 MLM 型 TAG。反应在 45 °C 下 120 小时内进行，使用来自Rhizomucor miehei (Lipozyme® ^{)的脂肪酶}RM 即时消息）。量化反应器中试剂的停留时间分布以评估反应器行为并诊断优先路径的存在。通过分析 TAG 组成来监测反应进程，在稳态下（反应 48 小时后），掺入度达到 39.91 ± 2.77%。为了增强癸酸的掺入，在相关的填充床反应器中进行酸解反应。结果表明，该生物催化剂具有良好的操作稳定性，填充床和相关填充床反应器的半衰期分别为 209.64 H、235.63 H，失活系数为 0.0061 H ^-1。