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Characterization of symbiotic and associated bacteria from entomopathogenic nematode Heterorhabditis sp. (nematode: Heterorhabditidae) isolated from India
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2020-12-07 , DOI: 10.1186/s41938-020-00343-9
Rashid Pervez , Showkat Ahmad Lone , Sasmita Pattnaik

Entomopathogenic nematodes (EPNs) harboring symbiotic bacteria are one of the safest alternatives to the chemical insecticides for the control of various insect pests. Infective juveniles of EPNs locate a target insect, enter through the openings, and reach the hemocoel, where they release the symbiotic bacteria and the target gets killed by the virulence factors of the bacteria. Photorhabdus with Heterorhabditis spp. are well documented; little is known about the associated bacteria. In this study, we explored the presence of symbiotic and associated bacteria from Heterorhabditis sp. (IISR-EPN 09) and characterized by phenotypic, biochemical, and molecular approaches. Six bacterial isolates, belonging to four different genera, were recovered and identified as follows: Photorhabdus luminescens, one each strain of Providencia vermicola, Pseudomonas entomophila, Alcaligenes aquatilis, and two strains of Alcaligenes faecalis based on the phenotypic, biochemical criteria and the sequencing of 16S rRNA gene. P. luminescens is symbiotically associated with Heterorhabditis sp. (IISR-EPN 09), whereas P. vermicola, P. entomophila, A. aquatilis, and A. faecalis are the associated bacteria. Further studies are needed to determine the exact role of the bacterial associates with the Heterorhabditis sp.

中文翻译:

昆虫病原线虫异种杆菌共生细菌和相关细菌的表征。(线虫:Heterorhabditidae)从印度分离

带有共生细菌的昆虫病原线虫(EPN)是控制各种害虫的化学杀虫剂的最安全替代品之一。EPNs的传染性幼虫定位目标昆虫,通过开口进入并到达血hemo,在那里它们释放出共生细菌,目标被细菌的毒力因子杀死。Photorhabdus与Herterhabditis spp。有据可查;对相关细菌知之甚少。在这项研究中,我们探讨了杂种猪共生菌和相关细菌的存在。(IISR-EPN 09),并具有表型,生化和分子方法的特点。回收了六个细菌分离物,它们分别属于四个不同属:根据表型,生化标准和16S rRNA基因的测序,假单胞菌(Pseudomonas entomophila),水产产碱杆菌(Alcaligenes aquatilis)和两个粪便产碱杆菌(Alcaligenes faecalis)菌株。P. luminescens与Heterorhabditis sp共生。(IISR-EPN 09),而腐生假单胞菌,嗜虫假单胞菌,水产曲霉和粪便曲霉是相关细菌。需要进一步的研究来确定细菌与杂种猪的细菌的确切作用。
更新日期:2020-12-07
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