Background: MiR-493 promotes the proliferation of prostate cancer (PC) cells by targeting PHLPP2. We aimed to explore the relationship between miR-493 and autophagy in PC.

Methods: qRT-PCR and western blotting were used to determine the mRNA levels and protein expression of miR-493, PHLPP2, autophagy gene BECN1 and ATG7 in PC cells. The autophagy gene expression was determined after PC cells transfected with miR-493 precursor or PHLPP2 precursor. Corresponding changes of autophagy phenotype and PC cell function were also studied.

Results: The mRNA levels and protein expression of miR-493, PHLPP2, BECN1 and ATG7 in PC cells were significantly decreased in PC cells. Overexpression of miR-493 or PHLPP2 markedly upregulated the expression levels of BECN1 and ATG7 in PC cells. Overexpression of miR-493 and PHLPP2 markedly promoted autophagy, and inhibited the invasion and cloning formation of PC cells.

Conclusion: MiR-493 is a potent inducer of cytotoxic autophagy that leads to prostate cancer inhibition by regulating on PHLPP2.

背景：MiR-493通过靶向PHLPP2促进前列腺癌细胞（PC）的增殖。我们旨在探讨miR-493与PC中自噬之间的关系。

方法：采用qRT-PCR和western blotting检测miR-493，PHLPP2，自噬基因BECN1和ATG7的mRNA水平和蛋白表达。在用miR-493前体或PHLPP2前体转染PC细胞后，确定自噬基因的表达。还研究了自噬表型和PC细胞功能的相应变化。

结果：PC细胞中miR-493，PHLPP2，BECN1和ATG7的mRNA水平和蛋白表达均明显降低。miR-493或PHLPP2的过表达显着上调了PC细胞中BECN1和ATG7的表达水平。miR-493和PHLPP2的过表达显着促进自噬，并抑制PC细胞的侵袭和克隆形成。

结论：MiR-493是细胞毒性自噬的有效诱导剂，可通过调节PHLPP2来抑制前列腺癌。