Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-12-06 , DOI: 10.1080/10799893.2020.1850784 Abulaiti Abula 1 , Guliayixiamu Saimaiti 2 , Xayimardan Maimaiti 1 , Wumitijiang Wuqikun 1 , Alimujiang Abulaiti 1 , Peng Ren 1 , Aihemaitijiang Yusufu 1
Abstract
Osteosarcoma (OS), a prevalent aggressive malignancy in the bone, has limited therapeutic targets and diagnostic biomarkers. In the current investigation, RT-qPCR showed that CDKN2B-AS1 was enhanced in OS samples and cells. This research was set to examine the modulation of CDKN2B-AS1 in OS. The expression of CDKN2B-AS1 and downstream molecules was analyzed by RT-qPCR method. CCK8, EdU staining along with Transwell assays were applied to evaluate cell proliferation and invasion. Those in vitro investigations specified that silencing of CDKN2B-AS1 with shRNAs obviously impeded the proliferation and invasion of MG63 cells. To authenticate the relationships between CDKN2B-AS1 and microRNA-122-5p (miR-122-5p) or cyclin G1 (CCNG1) and miR-122-5p, we next employed luciferase reporter assay. We displayed that CDKN2B-AS1 repressed miR-122-5p to restore CCNG1 expression. All in all, our findings substantiated the indispensable function of CDKN2B-AS1 in OS progression and the possible molecular mechanism.
中文翻译:
靶向microRNA-122/CCNG1轴的长链非编码RNA CDKN2B-AS1在骨肉瘤中的刺激作用
摘要
骨肉瘤 (OS) 是一种在骨骼中普遍存在的侵袭性恶性肿瘤,其治疗靶点和诊断生物标志物有限。在目前的调查中,RT-qPCR 显示 CDKN2B-AS1 在 OS 样本和细胞中得到增强。本研究旨在检查 OS 中 CDKN2B-AS1 的调制。通过 RT-qPCR 方法分析 CDKN2B-AS1 和下游分子的表达。CCK8、EdU 染色以及 Transwell 检测用于评估细胞增殖和侵袭。那些体外的研究表明,用 shRNA 沉默 CDKN2B-AS1 明显阻碍了 MG63 细胞的增殖和侵袭。为了验证 CDKN2B-AS1 和 microRNA-122-5p (miR-122-5p) 或细胞周期蛋白 G1 (CCNG1) 和 miR-122-5p 之间的关系,我们接下来采用荧光素酶报告基因分析。我们显示 CDKN2B-AS1 抑制 miR-122-5p 以恢复 CCNG1 表达。总而言之,我们的研究结果证实了 CDKN2B-AS1 在 OS 进展中不可或缺的功能以及可能的分子机制。