ABSTRACT The aim of this study was to prepare physically stable nanoemulsions containing three different essential oils (Rosemary, Cuminum, and Zataria Multiflora Boiss) by using two different nanoemulsification methods. Then microbial growth rate, antioxidant, and oxidant content of fresh fish fillet were assessed at 4°C and 10°C temperatures. Essential oils (EOs) were purified from Rosmarinus officinalis L., Zataria multiflora Boiss, and Cuminum cyminum L. and emulsified by two different methods: Ultrasonic Homogenization (USH) and Emulsion Phase Inversion (EPI). Samples of Acipenser stellatus (weight of 10 ± 1 g) were immersed in Nanoemulsified essential oils. Then antibacterial and antioxidant potential of these essential oils was analyzed by culturing in a tube and measuring PV and TBA, respectively, during 1, 3, 7, and 14 days after treatment. Cuminum cyminum L., at 4°C in the USH method reduced the growth of bacteria in the fish sample on all studied bacteria at 5% concentration compared to 3%. Also the results of Cuminum cyminum L. EO made by both methods used to emulsify the oil were better than the other two extracts. As well these results were observed for PV and TBA contents. This study showed that nanoemulsified Cuminum cyminum L. prepared by the USH method has good antioxidant potential and antibacterial effect among other essential oils and it can be used to produce novel natural antioxidants as well as flavoring agents that can be applied in different food products.

中文翻译：

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纳米乳生产技术提高纳米乳化精油对星蝽保存的生物活性潜力

摘要 本研究的目的是通过使用两种不同的纳米乳化方法制备含有三种不同精油（迷迭香、孜然和 Zataria Multiflora Boiss）的物理稳定纳米乳液。然后在 4°C 和 10°C 温度下评估新鲜鱼片的微生物生长速率、抗氧化剂和氧化剂含量。精油 (EO) 是从迷迭香、杂花杂草和孜然中提纯，并通过两种不同的方法乳化：超声波均质化 (USH) 和乳液相转化 (EPI)。将 Acipenser stellatus 样品（重量为 10 ± 1 g）浸入纳米乳化精油中。然后通过在试管中培养并分别测量 PV 和 TBA 来分析这些精油的抗菌和抗氧化潜力，分别在 1、3、7、和治疗后14天。在 USH 方法中，Cuminum cyminum L. 在 4°C 下减少了鱼样品中所有研究细菌的细菌生长，浓度为 5%，而浓度为 3%。此外，通过两种方法制备的 Cuminum cyminum L. EO 用于乳化油的结果优于其他两种提取物。同样，对于 PV 和 TBA 含量观察到这些结果。本研究表明，通过USH法制备的纳米乳化小茴香在其他精油中具有良好的抗氧化能力和抗菌作用，可用于生产新型天然抗氧化剂和调味剂，可应用于不同的食品。此外，通过两种方法制备的 Cuminum cyminum L. EO 用于乳化油的结果优于其他两种提取物。同样，对于 PV 和 TBA 含量观察到这些结果。本研究表明，通过USH法制备的纳米乳化小茴香在其他精油中具有良好的抗氧化能力和抗菌作用，可用于生产新型天然抗氧化剂和调味剂，可应用于不同的食品。此外，通过两种方法制备的 Cuminum cyminum L. EO 用于乳化油的结果优于其他两种提取物。同样，对于 PV 和 TBA 含量观察到这些结果。本研究表明，通过USH法制备的纳米乳化小茴香在其他精油中具有良好的抗氧化能力和抗菌作用，可用于生产新型天然抗氧化剂和调味剂，可应用于不同的食品。