Abstract

For centuries, Turkey has been a significant location here around 80 species of Hypericum with differing names widely occur, which is also known as Turkish folk medicine in treating some bacterial diseases as well as stomach and intestine inflammation. Recent studies have reported this herb family to contain numbers of bioactive compound contents. The study aims to investigate the protective effects of Hypericum triquetrifolium Turra (HT) upon oxidative stress and apoptosis in a rat model in which testes injury was induced by CP. The testicular injury was caused using CP (150 mg/kg). The rats were treated with a single dose (100 mg/kg) of methanol extract of HT to investigate various biochemical markers in the serum and plasma of blood samples apart from assessing the prognosis of CP-induced testicular damage. Added to that, histological analyses were performed to identify possible structural changes and apoptotic indicators, like Bax, Caspase-3, and Bcl-2. In CP Group, there was a rise in the levels of total oxidant status (TOS), malondialdehyde (MDA), oxidative stress index (OSI), Caspase-3, and Bax while superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), Bcl-2, and total antioxidant capacity (TAC) all decreased. Also, our histological analysis showed damaged testes. On the other hand, neither biochemical nor histological analysis showed testicular damage in HT Alone Group. In CP + HT Group, a significant number of the negatives changes due to CP were observed to have improved remarkably following an HT treatment. This study results suggest that HT could help improve CP-induced testicular injury thanks to its anti-oxidative and anti-apoptotic properties.

摘要

几个世纪以来，土耳其一直是这里的重要产地，大约有 80 种不同名称的金丝桃广泛分布，这也被称为土耳其民间医学，用于治疗一些细菌性疾病以及肠胃炎症。最近的研究报告说，这种草药家族含有大量的生物活性化合物。本研究旨在探讨金丝桃的保护作用。(HT) 在大鼠模型中氧化应激和细胞凋亡的影响，其中 CP 诱导睾丸损伤。使用 CP (150 mg/kg) 造成睾丸损伤。大鼠用单剂量（100 mg/kg）HT甲醇提取物处理，以研究血液样本血清和血浆中的各种生化标志物，同时评估CP诱导的睾丸损伤的预后。除此之外，还进行了组织学分析以确定可能的结构变化和凋亡指标，如 Bax、Caspase-3 和 Bcl-2。CP组总氧化状态（TOS）、丙二醛（MDA）、氧化应激指数（OSI）、Caspase-3和Bax水平升高，而超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽(GSH)、Bcl-2 和总抗氧化能力 (TAC) 均下降。还，我们的组织学分析显示睾丸受损。另一方面，生化和组织学分析均未显示单独HT组的睾丸损伤。在 CP + HT 组中，观察到大量由 CP 引起的负面变化在 HT 治疗后显着改善。该研究结果表明，由于其抗氧化和抗凋亡特性，HT 可以帮助改善 CP 引起的睾丸损伤。