Abstract

The aim of this study was to assess the impact of sulfamethoxazole (SMX) on oxidative stress indices in zebrafish (Danio rerio). The test was completed after 14 days. The tested concentrations were 50, 100 and 500 µg/L of SMX. Glutathione peroxidase, glutathione reductase, glutathione S-transferase and lipid peroxidation were investigated to determine the effects of SMX on oxidative stress in zebrafish. Lipid peroxidation gradually increased slightly (but non-significantly) at all tested concentrations during the test as compared to the control. The evaluation of oxidative stress biomarkers showed no significant changes in the activity of antioxidant enzymes in any experimental group exposed to SMX as compared to the control. The gradual increase in lipid peroxidation after 3 and 14 days in the SMX treated groups as compared to the control group indicates increasing cell membrane damage.

摘要

本研究的目的是评估磺胺甲恶唑 (SMX) 对斑马鱼 ( Danio rerio）。测试在14天后完成。测试的 SMX 浓度为 50、100 和 500 µg/L。研究了谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽 S-转移酶和脂质过氧化作用，以确定 SMX 对斑马鱼氧化应激的影响。与对照相比，在测试期间的所有测试浓度下，脂质过氧化逐渐略微（但不显着）增加。氧化应激生物标志物的评估表明，与对照组相比，暴露于 SMX 的任何实验组的抗氧化酶活性没有显着变化。与对照组相比，SMX 处理组在 3 天和 14 天后脂质过氧化的逐渐增加表明细胞膜损伤增加。