The molecular mechanism of high‐temperature stress (HTS) response, in plants, has so far been investigated using transcriptomics, while the dynamics of HTS‐responsive proteome remain unexplored. We examined the adaptive responses of the resilient wheat cultivar ‘Unnat Halna’ and dissected the HTS‐responsive proteome landscape. This led to the identification of 55 HTS‐responsive proteins (HRPs), which are predominantly involved in metabolism and defense pathways. Interestingly, HRPs included a 2‐cysteine peroxiredoxin (2CP), designated Ta2CP, presumably involved in stress perception and adaptation. Complementation of Ta2CP in yeast and heterologous expression in Arabidopsis demonstrated its role in thermotolerance. Both Ta2CP silencing and overexpression inferred the involvement of Ta2CP in plant growth and chlorophyll biosynthesis. We demonstrated that Ta2CP interacts with protochlorophyllide reductase b, TaPORB. Reduced TaPORB expression was found in Ta2cp‐silenced plants, while upregulation was observed in Ta2CP‐overexpressed plants. Furthermore, the downregulation of Ta2CP in Taporb‐silenced plants and reduction of protochlorophyllide in Ta2cp‐silenced plants suggested the key role of Ta2CP in chlorophyll metabolism. Additionally, the transcript levels of AGPase1 and starch were increased in Ta2cp‐silenced plants. More significantly, HTS‐treated Ta2cp‐silenced plants showed adaptive responses despite increased reactive oxygen species and peroxide concentrations, which might help in rapid induction of high‐temperature acclimation.

中文翻译：

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小麦2半胱氨酸过氧化物酶在叶绿素生物合成和适应高温中起双重作用

的分子机制ħ igh-吨emperature小号发束（HTS）反应，在植物中，迄今一直使用转录的调查，而HTS-响应蛋白质组的动态保持未知。我们检查了有弹性的小麦品种“ Unnat Halna”的适应性反应，并解剖了HTS反应性蛋白质组图。这导致鉴定出55种HTS反应蛋白（HRP），这些蛋白主要参与代谢和防御途径。有趣的是，HRP包括2-半胱氨酸过氧化物酶（2CP），称为Ta2CP，大概参与了压力感知和适应。Ta2CP在酵母中的互补和拟南芥中的异源表达证明了其在耐热性中的作用。两个都Ta2CP沉默和过度表达推断Ta2CP参与植物生长和叶绿素生物合成。我们证明了Ta2CP与原叶绿素还原酶b，TaPORB相互作用。在Ta2cp沉默的植物中发现TaPORB表达降低，而在Ta2CP过度表达的植物中观察到上调。此外，Tapob沉默的植物中Ta2CP的下调和Ta2cp沉默的植物中原叶绿素的减少表明Ta2CP在叶绿素代谢中起关键作用。此外，AGPase1的转录水平Ta2cp沉默的植物中淀粉和淀粉含量增加。更重要的是，经过HTS处理的Ta2cp沉默的植物显示了适应性反应，尽管活性氧和过氧化物的浓度增加了，这可能有助于快速诱导高温驯化。