Abstract This study was conducted in order to determine the effects of exogenous selenium (Se) supply (10 and 30 mg L−1 Na2SeO4) on the resistance to salt stress (NaCl 200 mM) of olive (Olea europaea L.) cv. Arbequina grown in hydroponic culture. Vegetative indices were monitored: plant growth, photosynthesis, leaf gas exchange and relative water and chlorophyll content. The bio-molecular parameters relative to proline, total Se in the leaves, and the quantity of proline, SeO42- and Ca2+ released by the roots were also monitored. The present study describes for the first time the monitoring of proline, Se and Ca2+ released from the roots and their content in leaves after treatment with selenate in olive trees grown in hydroponic culture and subjected or not to salt stress. The results showed that NaCl partially reduced the absorption of selenate in the Arbequina cultivar. Despite this, treatment with Na-selenate in salt stressed plants stabilized the levels of proline in the leaves and reduced the loss of this osmolyte from the roots. Selenate also promoted the release of Ca2+ from the roots, thus contributing to the restoration of ionic homeostasis. The positive effects of Se on the monitored bio-molecules were also confirmed by the improvement of vegetative indices dramatically altered bystrong salt stress. Indeed, treatment with selenate helped to reduce the amount of toxic effects on leaf gas exchange, improving photosynthesis, and mitigated the reduction of leaf dry weight and relative water content of the leaves.

中文翻译：

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盐胁迫条件下补硒对橄榄的影响

摘要 本研究旨在确定外源硒 (Se) 供应（10 和 30 mg L-1 Na2SeO4）对橄榄 (Olea europaea L.) cv. 抗盐胁迫 (NaCl 200 mM) 的影响。Arbequina 在水培培养中生长。监测营养指数：植物生长、光合作用、叶片气体交换和相对水分和叶绿素含量。还监测了与脯氨酸相关的生物分子参数、叶片中的总硒以及根部释放的脯氨酸、SeO42- 和 Ca2+ 的数量。本研究首次描述了对水培栽培橄榄树中的脯氨酸、Se 和 Ca2+ 及其在用硒酸盐处理后的叶子中释放的脯氨酸、Se 和 Ca2+ 进行的监测，这些橄榄树是否受到盐胁迫。结果表明，NaCl 部分降低了 Arbequina 品种对硒酸盐的吸收。尽管如此，在盐胁迫的植物中用硒酸钠处理稳定了叶子中脯氨酸的水平，并减少了这种渗透物从根部的损失。硒酸盐还促进了根部 Ca2+ 的释放，从而有助于恢复离子稳态。Se 对监测到的生物分子的积极影响也通过强盐胁迫显着改变植物指数的改善得到证实。事实上，用硒酸盐处理有助于减少对叶片气体交换的毒性作用，改善光合作用，并减轻叶片干重和相对水分含量的降低。在盐胁迫的植物中用硒酸钠处理稳定了叶子中脯氨酸的水平，并减少了这种渗透物从根部的损失。硒酸盐还促进了根部 Ca2+ 的释放，从而有助于恢复离子稳态。Se 对监测到的生物分子的积极影响也通过强盐胁迫显着改变植物指数的改善得到证实。事实上，用硒酸盐处理有助于减少对叶片气体交换的毒性作用，改善光合作用，并减轻叶片干重和相对水分含量的降低。在盐胁迫的植物中用硒酸钠处理稳定了叶子中脯氨酸的水平，并减少了这种渗透物从根部的损失。硒酸盐还促进了根部 Ca2+ 的释放，从而有助于恢复离子稳态。Se 对监测到的生物分子的积极影响也通过强盐胁迫显着改变植物指数的改善得到证实。事实上，用硒酸盐处理有助于减少对叶片气体交换的毒性作用，改善光合作用，并减轻叶片干重和相对水分含量的降低。Se 对监测到的生物分子的积极影响也通过强盐胁迫显着改变植物指数的改善得到证实。事实上，用硒酸盐处理有助于减少对叶片气体交换的毒性作用，改善光合作用，并减轻叶片干重和相对水分含量的降低。Se 对监测到的生物分子的积极影响也通过强盐胁迫显着改变植物指数的改善得到证实。事实上，用硒酸盐处理有助于减少对叶片气体交换的毒性作用，改善光合作用，并减轻叶片干重和相对水分含量的降低。