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Light contributes to salt resistance through GAI protein regulation in Arabidopsis thaliana
Plant Physiology and Biochemistry ( IF 6.5 ) Pub Date : 2020-12-07 , DOI: 10.1016/j.plaphy.2020.12.004
Saima Arain , Maria Meer , Muhammad Sajjad , Humaira Yasmin

The role of DELLAs in response to light intensity under salt stress is largely unknown. Therefore, the effect of three light intensities-low (35), medium (60), and high (155) μmol m−2 s−1 on Arabidopsis plants growth under saline condition (150 mM NaCl) was evaluated. High light intensity exhibited significant growth in the number of lateral roots related to the low light. Immunoblot assay revealed increased DELLA accumulation at the seedling stage under high light intensity. High light promotes seed germination by 24–44%, whilst, lateral roots by 25–90% in wild-type ecotypes. The lateral roots increased significantly in gai (gibberellic acid insensitive mutant) as compared with gai-t6 (wild type like gibberellic acid insensitive mutant) in response to low to medium and high to medium light intensity. High light increased seedling survival rate by 67% in Col-0 (Columbia) and 60% in Ler (Landsberg erecta) and showed a 28% increase in survival rate in gai mutant under salt stress as compared to gai-t6. Furthermore, salt-stress responsive genes’ expression in gai-mutant establishes the relationship of DELLA proteins with salt resistance. Together, light is a cardinal element, its optimum quantity is highly beneficial and promotes salt stress resistance through DELLA protein at seedling stage in plants.



中文翻译:

光通过拟南芥中的GAI蛋白调节作用增强了耐盐性

DELLAs在盐胁迫下响应光强度的作用在很大程度上是未知的。因此,评价了在盐水条件(150mM NaCl)下低(35),中(60)和高(155)μmolm -2 s -1的三种强度对拟南芥植物生长的影响。高光强度显示与低光有关的侧根数量显着增加。免疫印迹分析显示,在高光强度下,苗期DELLA积累增加。在野生型生态型中,强光可促进种子发芽24-44%,而侧根可促进25-90%。侧根在显著增加(赤霉酸不敏感的突变体)与比较改T6(野生型,如赤霉素不敏感突变体)对低到中等和高到中等光强度的响应。高光使Col-0(哥伦比亚)的幼苗成活率提高67%,而L er(Landsberg erecta)的成活率提高60%,与gai-t6相比,盐胁迫下gai突变体的成活率提高了28%。此外,盐胁迫响应基因在盖氏突变体中的表达建立了DELLA蛋白与抗盐性的关系。总之,光是最基本的元素,它的最佳数量是非常有益的,并且可以在植物的幼苗阶段通过DELLA蛋白促进抗盐胁迫。

更新日期:2020-12-10
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