Boronic acids are important ligands used to selectively recognize and enrich compounds containing cis-diol groups such as nucleosides. In the present study, organic-inorganic hybrid [POSS-MAH-BPA] monolithic column was prepared for the first time in the literature as a new boronate affinity system for the recognition of nucleosides. The selectivity of the [POSS-MAH-PBA] boronate affinity monolithic column for the recognition of cis-diol containing adenosine nucleoside from its analogue molecule of deoxyadenosine triphosphate, dATP, non-cis-diol containing compound was investigated both by UV and HPLC studies. When the relative selectivity coefficients are compared, the [POSS-MAH-PBA] boronate affinity monolithic column is 4.25 times more selective for adenosine than [POSS-MAH] monolithic column. Besides, to determine endogenous nucleosides in biological fluids, which may serve as non-invasive cancer biomarkers, nucleosides were spiked into the urine solutions and passed through the [POSS-MAH-PBA] boronate affinity monolithic column, and the nucleosides were confirmed by HPLC. The adenosine recognition capability of the [POSS-MAH-PBA] boronate affinity monolithic column with an average enrichment factor of 48.9-fold was apparently superior to that of the [POSS-MAH] monolithic column.

Methacryl Polyhedral Oligomeric Silsesquioxanes (POSS-MA) with nano-sized stable 3-dimensional architectures provided the advantage of being used as an adsorbent for the monolithic structure by providing high surface area, 507.60 m²/g, and enabling vinyl groups to function with amino acid-based MAH monomers capable of providing electrons to coordinate PBA. Recovery results of more than 90% for adenosine showed that the [POSS-MAH-PBA] boronate affinity monolithic column could be a promising adsorbent for selective adsorption of cis-diol containing compounds such as nucleosides.

硼酸是重要的配体，用于选择性识别和富集含有顺式二醇基团的化合物（如核苷）。在本研究中，有机-无机杂化[POSS-MAH-BPA]整体柱在文献中首次制备为用于识别核苷的新型硼酸酯亲和系统。通过UV和HPLC研究，研究了[POSS-MAH-PBA]硼酸酯亲和整体柱从其脱氧腺苷三磷酸，dATP，不含顺式二醇化合物的类似物分子识别含顺式二醇的腺苷核苷的选择性。 。当比较相对选择性系数时，[POSS-MAH-PBA]硼酸酯亲和整体柱对腺苷的选择性是[POSS-MAH]整体柱的4.25倍。除了，为了确定生物液体中的内源性核苷（可作为非侵入性癌症生物标记物），将核苷掺入尿液中并通过[POSS-MAH-PBA]硼酸酯亲和整体柱，并通过HPLC确认核苷。[POSS-MAH-PBA]硼酸酯亲和整体柱的腺苷识别能力平均富集因子为48.9倍，明显优于[POSS-MAH-PH]整体柱。

具有纳米级稳定的3维结构的甲基丙烯酸多面体低聚倍半硅氧烷（POSS-MA）具有以下优点：提供了507.60 m ² / g的高表面积，使乙烯基能够与单官能团结构一起用作吸附剂氨基酸基MAH单体，能够提供电子来协调PBA。腺苷的回收率超过90％，表明[POSS-MAH-PBA]硼酸酯亲和整体柱可能是一种有希望的吸附剂，可选择性吸附含顺式二醇的化合物（如核苷）。