Listeria monocytogenes is an important food-borne pathogen and its bacteriophages are promising tools for its control in food and surfaces. Listeria bacteriophages belonging to the genus Pecentumvirus of the family Herelleviridae are strictly lytic, have a contractile tail and a large double stranded DNA genome (mean of 135.4 kb). We report the isolation and genome sequences of two new Pecentumvirus bacteriophages: vB_Lino_VEfB7 and vB_Liva_VAfA18. Twenty-one bacteriophages of this genus have been described and their genomes were used for the study of Pecentumvirus evolution. Analyses showed collinear genomes and gene gain and loss propensity and recombination events were distinctly found in two regions. A large potential recombination event (≈20 kB) was detected in P100 and vB_Liva_VAfA18. Phylogenetic analyses of multi-gene alignments showed that diversification events formed two groups of species distantly related.

单核细胞增生李斯特菌是一种重要的食源性病原体，其噬菌体是控制其在食品和表面的有前途的工具。李斯特菌噬菌体属于海瑞氏病毒科的百分体病毒属，具有严格的溶解性，具有可收缩的尾部和大的双链 DNA 基因组（平均 135.4 kb）。我们报告了两种新的Pecentumvirus噬菌体的分离和基因组序列：vB_Lino_VEfB7 和 vB_Liva_VAfA18。已经描述了该属的 21 个噬菌体，它们的基因组被用于研究Pecentumvirus进化。分析表明，在两个区域中明显发现了共线基因组和基因增益和丢失倾向以及重组事件。在 P100 和 vB_Liva_VAfA18 中检测到一个大的潜在重组事件 (≈20 kB)。多基因比对的系统发育分析表明，多样化事件形成了两组远亲的物种。