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Heterogeneous biocatalytical esterification by recombinant Thermomyces lanuginosus lipase immobilized on macroporous carbon aerogel
Catalysis Today ( IF 5.3 ) Pub Date : 2020-12-07 , DOI: 10.1016/j.cattod.2020.11.018
Galina Kovalenko , Larisa Perminova , Anatoly Beklemishev

Heterogeneous biocatalysts prepared by immobilizing recombinant lipases are promising for implementation in organic synthesis of various esters used as fragrances, emollients, emulsifiers, non-ionic surfactants, plasticizers, etc. For this research, the Thermomyces lanuginosus lipase produced by the authoring recombinant Pichia pastoris strain was adsorbed on the authoring macroporous carbon aerogel. The prepared biocatalysts were studied in the periodic processes of enzymatic esterification carried out in nonconventional anhydrous media of organic solvents at ambient conditions (22 ± 2 °C, 1 bar). Biocatalytical activities depended strongly on both the selected pair of lipase substrates (acid and alcohol) involved in reaction and the organic solvent used for esterification. The reaction rates of fatty C7 acid esterification with double and triple bond C3 alcohols, such as allyl and propargyl alcohols, were found to be 2–4 times lower than with aliphatic propanol. Isomerism of an acid (not alcohol) molecule greatly affected the esterification rates. It was observed that isomers of C4 and C5 acids (iso-butyric and iso-valeric) practically did not react with butanol. The esterification rates strongly depended on the polarity of the organic solvent characterized by the parameter logP. With an increase in the solvent polarity from non-polar hexane to polar acetone, the esterification rate decreased by two orders of magnitude. The prepared lipase-active biocatalysts had a sufficiently high operational stability under the chosen optimal reaction conditions; their stationary activity (300 ± 100 U·g–1) did not change in periodic reaction cycles during several tens of hours.



中文翻译:

固定在大孔碳气凝胶上的重组绵状嗜热丝孢菌脂肪酶的多相生物催化酯化

通过固定重组脂肪酶制备的多相生物催化剂有望用于各种酯的有机合成,用作香料、润肤剂、乳化剂、非离子表面活性剂、增塑剂等。 在这项研究中,作者重组毕赤酵母生产的疏毛嗜热菌脂肪酶菌株被吸附在创作的大孔碳气凝胶上。在环境条件 (22 ± 2 °C, 1 bar) 下,在有机溶剂的非常规无水介质中进行的酶促酯化的周期性过程中研究了制备的生物催化剂。生物催化活性强烈依赖于所选择的参与反应的脂肪酶底物对(酸和醇)和用于酯化的有机溶剂。发现 C7 脂肪酸与双键和三键 C3 醇(如烯丙醇和炔丙醇)的酯化反应速率比脂肪族丙醇低 2-4 倍。酸(不是醇)分子的异构现象极大地影响了酯化速率。据观察,的C4和C5的异构体(丁酸和-戊酸)实际上不与丁醇反应。酯化速率很大程度上取决于由参数 logP 表征的有机溶剂的极性。随着溶剂极性从非极性己烷到极性丙酮的增加,酯化率下降了两个数量级。制备的脂肪酶活性生物催化剂在选定的最佳反应条件下具有足够高的操作稳定性;它们的固定活性 (300 ± 100 U·g –1 ) 在几十小时的周期性反应循环中没有变化。

更新日期:2020-12-07
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