Bacterial drug resistance poses a global challenge to established antibacterial prevention and treatment schemes. Recent approaches focus on raising algal extracts' efficacy by fusing them to other antibacterial agents employing nanotechnology. Here, we successfully biosynthesized AgNPs using Galaxaura rugosa crude methanol extract (AgNP_CR) and raw powder aqueous (AgNP_AQ) solution. The resultant AgNPs were characterized using UV-visible spectroscopy, Fourier-transform infrared spectroscopy, dynamic light scattering, and transmission electron microscopy. The AgNPs were spherical and ranged between 60 to 114.4 nm. The presence of the hydroxyl group and alkenes propped AgNPs biosynthesis. We investigated AgNPs antibacterial and antibiofilm activity against eighteen medically important bacteria, including multidrug-resistant bacteria. The sensitive and resistant Acinetobacter baumannii and Staphylococcus aureus were the most affected strains. The biofilm of MDR strains was mostly disturbed, indicating the reduced bacterial fitness in resistant strains. These results may aid antibacterial agents' development by investing in natural resources available abundantly in our environment.

细菌耐药性对既定的抗菌素预防和治疗方案提出了全球性挑战。最近的方法集中于通过使用纳米技术将藻类提取物融合到其他抗菌剂中来提高其功效。在这里，我们成功地使用了芦荟粗甲醇提取物（AgNP _CR）和水性原料粉（AgNP _AQ）生物合成了AgNP。）解决方案。使用紫外可见光谱，傅立叶变换红外光谱，动态光散射和透射电子显微镜对所得的AgNP进行表征。AgNP是球形的，范围在60至114.4nm之间。羟基和烯烃的存在促进了AgNPs的生物合成。我们研究了AgNPs对18种医学上重要的细菌（包括耐多药细菌）的抗菌和生物膜活性。敏感和耐药的鲍曼不动杆菌和金黄色葡萄球菌是受影响最大的菌株。MDR菌株的生物膜大部分受到干扰，表明耐药菌株的细菌适应性降低。这些结果可通过投资我们环境中大量可用的自然资源来帮助抗菌剂的发展。