Abstract

Flonicamid is a novel, selective, systemic pyridinecarboxamide insecticide that effectively controls hemipterous pests. Sulfoxaflor, a sulfoximine insecticide, effectively controls many sap-feeding insect pests. Ensifer meliloti CGMCC 7333 transforms flonicamid into N-(4-trifluoromethylnicotinoyl) glycinamide (TFNG-AM). Resting cells of E. meliloti CGMCC 7333 (optical density at 600 nm [OD₆₀₀] = 5) transformed 67.20% of the flonicamid in a 200-mg/L solution within 96 h. E. meliloti CGMCC 7333 transforms sulfoxaflor into N-(methyl(oxido){1-[6-(trifluoromethyl) pyridin-3-yl] ethyl}-k4-sulfanylidene) urea (X11719474). E. meliloti CGMCC 7333 resting cells (OD₆₀₀ = 5) transformed 89.36% of the sulfoxaflor in a 200 mg/L solution within 96 h. On inoculating 2 mL of E. meliloti CGMCC 7333 (OD₆₀₀ = 10) into soil containing 80 mg/kg flonicamid, 91.1% of the flonicamid was transformed within 9 d (half-life 2.6 d). On inoculating 2 mL of E. meliloti CGMCC 7333 (OD₆₀₀ = 10) into soil containing 80 mg/kg sulfoxaflor, 83.9% of the sulfoxaflor was transformed within 9 d (half-life 3.4 d). Recombinant Escherichia coli harboring the E. meliloti CGMCC 7333 nitrile hydratase (NHase)-encoding gene and NHase both showed the ability to transform flonicamid or sulfoxaflor into their corresponding amides, TFNG-AM and X11719474, respectively. These findings may help develop a bioremediation agent for the elimination of flonicamid and sulfoxaflor contamination.

摘要

氟尼卡米是一种新型的，选择性的，系统性吡啶甲酰胺类杀虫剂，可有效控制半虫害。磺胺嘧啶杀虫剂磺胺草醚可有效控制许多以树液为食的害虫。蜜蜂（Ensifer meliloti） CGMCC 7333将氟尼卡胺转化为N-（4-三氟甲基烟酰）甘氨酰胺（TFNG-AM）。大肠杆菌E. meliloti CGMCC 7333（在600 nm [OD ₆₀₀ ] = 5处的光密度）的静息细胞在96 h内将200 mg / L溶液中的67.20％的氟尼考定转化。大肠杆菌（E.meliloti）CGMCC 7333将磺胺草碱转化为N-（甲基（氧化）{1- [6-（6-三氟甲基）吡啶-3-基]乙基} -k4-亚磺酰基）脲（X11719474）。大肠杆菌E.meliloti CGMCC 7333静止细胞（OD₆₀₀ = 5）在96小时内在200 mg / L的溶液中转化了89.36％的亚磺草胺。向含有80 mg / kg氟尼草胺的土壤中接种2 mL大肠杆菌E. CGMCC 7333（OD ₆₀₀ = 10）后，在9天内转化了91.1％的氟尼草胺（半衰期为2.6 d）。向含有80 mg / kg磺胺草隆的土壤中接种2 mL苜蓿芽孢杆菌CGMCC 7333（OD ₆₀₀ = 10）后，在9 d内转化了83.9％的磺胺草碱（半衰期3.4 d）。重组大肠杆菌中窝藏E.根瘤菌CGMCC 7333腈水合酶（NHase）编码基因和NHase均显示出将氟尼草胺或磺胺草胺分别转化为其相应的酰胺TFNG-AM和X11719474的能力。这些发现可能有助于开发一种生物修复剂，以消除氟尼卡米和磺胺草醚污染。